Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Sung Eun Kim, Young Pil Yun, Kyu Sik Shim, Kyeongsoon Park, Sung Wook Choi, Dong Hyup Shin, Dong Hun Suh

Research output: Contribution to journalArticle

37 Citations (Scopus)


The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 2015 Oct 1



  • Bone morphogenic protein-2 (BMP-2)
  • Bone tissue engineering
  • Fluidic device
  • Heparin
  • Porous microspheres

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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