Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

Sung Eun Kim, Young Pil Yun, Kyu Sik Shim, Kyeongsoon Park, Sung Wook Choi, Dong Hyup Shin, Dong Hun Suh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume134
DOIs
Publication statusPublished - 2015 Oct 1

Fingerprint

Immobilized Proteins
heparins
tissue engineering
Tissue Engineering
Microspheres
Tissue engineering
bones
Heparin
Bone
proteins
Proteins
Bone and Bones
Fabrication
fabrication
osteocalcin
dopamine
phosphatases
Photoelectron Spectroscopy
Osteopontin
Osteocalcin

Keywords

  • Bone morphogenic protein-2 (BMP-2)
  • Bone tissue engineering
  • Fluidic device
  • Heparin
  • Porous microspheres

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering. / Kim, Sung Eun; Yun, Young Pil; Shim, Kyu Sik; Park, Kyeongsoon; Choi, Sung Wook; Shin, Dong Hyup; Suh, Dong Hun.

In: Colloids and Surfaces B: Biointerfaces, Vol. 134, 01.10.2015, p. 453-460.

Research output: Contribution to journalArticle

Kim, Sung Eun ; Yun, Young Pil ; Shim, Kyu Sik ; Park, Kyeongsoon ; Choi, Sung Wook ; Shin, Dong Hyup ; Suh, Dong Hun. / Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering. In: Colloids and Surfaces B: Biointerfaces. 2015 ; Vol. 134. pp. 453-460.
@article{5583c1a5b7374ead9882e1644042da59,
title = "Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering",
abstract = "The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.",
keywords = "Bone morphogenic protein-2 (BMP-2), Bone tissue engineering, Fluidic device, Heparin, Porous microspheres",
author = "Kim, {Sung Eun} and Yun, {Young Pil} and Shim, {Kyu Sik} and Kyeongsoon Park and Choi, {Sung Wook} and Shin, {Dong Hyup} and Suh, {Dong Hun}",
year = "2015",
month = "10",
day = "1",
doi = "10.1016/j.colsurfb.2015.05.003",
language = "English",
volume = "134",
pages = "453--460",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

}

TY - JOUR

T1 - Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering

AU - Kim, Sung Eun

AU - Yun, Young Pil

AU - Shim, Kyu Sik

AU - Park, Kyeongsoon

AU - Choi, Sung Wook

AU - Shin, Dong Hyup

AU - Suh, Dong Hun

PY - 2015/10/1

Y1 - 2015/10/1

N2 - The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

AB - The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects.

KW - Bone morphogenic protein-2 (BMP-2)

KW - Bone tissue engineering

KW - Fluidic device

KW - Heparin

KW - Porous microspheres

UR - http://www.scopus.com/inward/record.url?scp=84938071385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938071385&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2015.05.003

DO - 10.1016/j.colsurfb.2015.05.003

M3 - Article

VL - 134

SP - 453

EP - 460

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -