Fabrication of a new tubular fibrous PLCL scaffold for vascular tissue engineering

Sang Heon Kim, Jae Hyun Kwon, Min Sub Chung, Eunna Chung, Youngmee Jung, Soo Hyun Kim, Young Ha Kim

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Biodegradable macroporous scaffolds have been developed for tissue-engineering applications. We fabricated and characterized a new tubular, macroporous, fibrous scaffold using a very elastic biodegradable co-polymer, poly(L-lactide-co-caprolactone) (PLCL, 5:5) in a gel-spinning process. A viscous PLCL solution was spun as a gel-phase under swirl-flow conditions and was subsequently fabricated to produce a tubular fibrous scaffold on a rotating cylindrical shaft in a methanol solution. The porosity and median pore size of the fibrous PLCL scaffolds were 55-75% and 120-150 μm, respectively, using a 5-10% PLCL solution. The use of a 7.5% (w/v) solution resulted in scaffolds with tensile strength and elastic modulus of 3.39 MPa and 1.22 MPa, respectively. The scaffolds exhibited 500-600% elongation-at-break. The tensile strength and modulus of fibrous PLCL scaffolds were proven to decrease on lowering the concentration of the PLCL spinning solution; however, the tensile strength and modulus of fibrous PLCL scaffolds, produced from 5% solutions, are approximately 4-and 5-times higher than those of extruded PLCL scaffolds. These properties indicated that the fibrous PLCL scaffolds were very elastic and mechanically strong. The scaffolds appeared to be well interconnected between the pores as determined by SEM imaging analysis. In addition, the cell-seeding efficiency was 2-fold higher using gel-spun scaffolds than using extruded scaffolds. These results suggest that the gel-spun fibrous PLCL scaffold is an excellent matrix for vascular tissue-engineering applications.

Original languageEnglish
Pages (from-to)1359-1374
Number of pages16
JournalJournal of Biomaterials Science, Polymer Edition
Volume17
Issue number12
DOIs
Publication statusPublished - 2006 Dec

Keywords

  • Fibrous scaffold
  • Gel spinning
  • Poly(L-lactide-co-caprolactone)
  • Vascular tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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  • Cite this

    Kim, S. H., Kwon, J. H., Chung, M. S., Chung, E., Jung, Y., Kim, S. H., & Kim, Y. H. (2006). Fabrication of a new tubular fibrous PLCL scaffold for vascular tissue engineering. Journal of Biomaterials Science, Polymer Edition, 17(12), 1359-1374. https://doi.org/10.1163/156856206778937244