Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells

Yong Kwan Noh, Avelino Dos Santos Da Costa, Yong Seek Park, Ping Du, Ik Hwan Kim, Kwideok Park

Research output: Contribution to journalArticle

Abstract

Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.

Original languageEnglish
Pages (from-to)210-218
Number of pages9
JournalCarbohydrate Polymers
Volume219
DOIs
Publication statusPublished - 2019 Sep 1

Fingerprint

Scaffolds (biology)
Stem cells
Collagen
Scaffolds
Cellulose
Fabrication
Composite materials
Bone
Integrin-Binding Sialoprotein
Tissue Scaffolds
Tissue regeneration
Osteocalcin
Collagen Type I
Muscle
Actins
Calcium
Blood
Proteins
Water

Keywords

  • Bacterial cellulose (BC)
  • Bone regeneration
  • Collagen
  • Osteogenesis
  • Umbilical cord blood derived mesenchymal stem cells (UCB-MSCs)

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells. / Noh, Yong Kwan; Dos Santos Da Costa, Avelino; Park, Yong Seek; Du, Ping; Kim, Ik Hwan; Park, Kwideok.

In: Carbohydrate Polymers, Vol. 219, 01.09.2019, p. 210-218.

Research output: Contribution to journalArticle

Noh, Yong Kwan ; Dos Santos Da Costa, Avelino ; Park, Yong Seek ; Du, Ping ; Kim, Ik Hwan ; Park, Kwideok. / Fabrication of bacterial cellulose-collagen composite scaffolds and their osteogenic effect on human mesenchymal stem cells. In: Carbohydrate Polymers. 2019 ; Vol. 219. pp. 210-218.
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