Fabrication of disposable protein chip for simultaneous sample detection

Chang S. Lee, Sang H. Lee, Yun G. Kim, Min-Kyu Oh, Taek Sung Hwang, Young Woo Ree, Hwan Moon Song, Bo Yeol Kim, Yong Kweon Kim, Byung G. Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, we have described a method for the fabrication of a protein chip on silicon substrate using hydrophobic thin film and microfluidic channels, for the simultaneous detection of multiple targets in samples. The use of hydrophobic thin film provides for a physical, chemical, and biological barrier for protein patterning. The microfluidic channels create four protein patterned strips on the silicon surfaces with a high signal-to-noise ratio. The feasibility of the protein chips was determined in order to discriminate between each protein interaction in a mixture sample that included biotin, ovalbumin, hepatitis B antigen, and hepatitis C antigen. In the fabrication of the multiplexed assay system, the utilization of the hydrophobic thin film and the microfluidic networks constitutes a more convenient method for the development of biosensors or biochips. This technique may be applicable to the simultaneous evaluation of multiple protein-protein interactions.

Original languageEnglish
Pages (from-to)455-461
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number5
DOIs
Publication statusPublished - 2006 Sep 1

Fingerprint

Protein Array Analysis
Microfluidics
Proteins
Fabrication
Silicon
Hepatitis C Antigens
Antigens
Hepatitis B Antigens
Thin films
Ovalbumin
Signal-To-Noise Ratio
Biosensing Techniques
Biotin
Biochips
Biosensors
Assays
Signal to noise ratio
Substrates

Keywords

  • Microchannel
  • Protein chip
  • Protein patterning
  • Simultaneous detection

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology

Cite this

Lee, C. S., Lee, S. H., Kim, Y. G., Oh, M-K., Hwang, T. S., Ree, Y. W., ... Kim, B. G. (2006). Fabrication of disposable protein chip for simultaneous sample detection. Biotechnology and Bioprocess Engineering, 11(5), 455-461. https://doi.org/10.1007/BF02932314

Fabrication of disposable protein chip for simultaneous sample detection. / Lee, Chang S.; Lee, Sang H.; Kim, Yun G.; Oh, Min-Kyu; Hwang, Taek Sung; Ree, Young Woo; Song, Hwan Moon; Kim, Bo Yeol; Kim, Yong Kweon; Kim, Byung G.

In: Biotechnology and Bioprocess Engineering, Vol. 11, No. 5, 01.09.2006, p. 455-461.

Research output: Contribution to journalArticle

Lee, CS, Lee, SH, Kim, YG, Oh, M-K, Hwang, TS, Ree, YW, Song, HM, Kim, BY, Kim, YK & Kim, BG 2006, 'Fabrication of disposable protein chip for simultaneous sample detection', Biotechnology and Bioprocess Engineering, vol. 11, no. 5, pp. 455-461. https://doi.org/10.1007/BF02932314
Lee, Chang S. ; Lee, Sang H. ; Kim, Yun G. ; Oh, Min-Kyu ; Hwang, Taek Sung ; Ree, Young Woo ; Song, Hwan Moon ; Kim, Bo Yeol ; Kim, Yong Kweon ; Kim, Byung G. / Fabrication of disposable protein chip for simultaneous sample detection. In: Biotechnology and Bioprocess Engineering. 2006 ; Vol. 11, No. 5. pp. 455-461.
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