Fabrication of disposable protein chip for simultaneous sample detection

Chang Soo Lee, Sang Ho Lee, Yun Gon Kim, Min Kyu Oh, Taek Sung Hwang, Young Woo Ree, Hwan Moon Song, Bo Yeol Kim, Yong Kweon Kim, Byung Gee Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study, we have described a method for the fabrication of a protein chip on silicon substrate using hydrophobic thin film and microfluidic channels, for the simultaneous detection of multiple targets in samples. The use of hydrophobic thin film provides for a physical, chemical, and biological barrier for protein patterning. The microfluidic channels create four protein patterned strips on the silicon surfaces with a high signal-to-noise ratio. The feasibility of the protein chips was determined in order to discriminate between each protein interaction in a mixture sample that included biotin, ovalbumin, hepatitis B antigen, and hepatitis C antigen. In the fabrication of the multiplexed assay system, the utilization of the hydrophobic thin film and the microfluidic networks constitutes a more convenient method for the development of biosensors or biochips. This technique may be applicable to the simultaneous evaluation of multiple protein-protein interactions.

Original languageEnglish
Pages (from-to)455-461
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number5
DOIs
Publication statusPublished - 2006 Sep

Keywords

  • Microchannel
  • Protein chip
  • Protein patterning
  • Simultaneous detection

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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  • Cite this

    Lee, C. S., Lee, S. H., Kim, Y. G., Oh, M. K., Hwang, T. S., Ree, Y. W., Song, H. M., Kim, B. Y., Kim, Y. K., & Kim, B. G. (2006). Fabrication of disposable protein chip for simultaneous sample detection. Biotechnology and Bioprocess Engineering, 11(5), 455-461. https://doi.org/10.1007/BF02932314