Fabrication of enzyme-based coatings on intact multi-walled carbon nanotubes as highly effective electrodes in biofuel cells

Byoung Chan Kim, Inseon Lee, Seok Joon Kwon, Youngho Wee, Ki Young Kwon, Chulmin Jeon, Hyo Jin An, Hee Tae Jung, Su Ha, Jonathan S. Dordick, Jungbae Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

CNTs need to be dispersed in aqueous solution for their successful use, and most methods to disperse CNTs rely on tedious and time-consuming acid-based oxidation. Here, we report the simple dispersion of intact multi-walled carbon nanotubes (CNTs) by adding them directly into an aqueous solution of glucose oxidase (GOx), resulting in simultaneous CNT dispersion and facile enzyme immobilization through sequential enzyme adsorption, precipitation, and crosslinking (EAPC). The EAPC achieved high enzyme loading and stability because of crosslinked enzyme coatings on intact CNTs, while obviating the chemical pretreatment that can seriously damage the electron conductivity of CNTs. EAPC-driven GOx activity was 4.5- and 11-times higher than those of covalently-attached GOx (CA) on acid-treated CNTs and simply-adsorbed GOx (ADS) on intact CNTs, respectively. EAPC showed no decrease of GOx activity for 270 days. EAPC was employed to prepare the enzyme anodes for biofuel cells, and the EAPC anode produced 7.5-times higher power output than the CA anode. Even with a higher amount of bound non-conductive enzymes, the EAPC anode showed 1.7-fold higher electron transfer rate than the CA anode. The EAPC on intact CNTs can improve enzyme loading and stability with key routes of improved electron transfer in various biosensing and bioelectronics devices.

Original languageEnglish
Article number40202
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017 Jan 5

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Biological fuel cells
Carbon Nanotubes
Fabrication
Coatings
Electrodes
Crosslinking
Enzymes
Glucose Oxidase
Adsorption
Anodes
Electrons
Enzyme immobilization
Acids

ASJC Scopus subject areas

  • General

Cite this

Fabrication of enzyme-based coatings on intact multi-walled carbon nanotubes as highly effective electrodes in biofuel cells. / Kim, Byoung Chan; Lee, Inseon; Kwon, Seok Joon; Wee, Youngho; Kwon, Ki Young; Jeon, Chulmin; An, Hyo Jin; Jung, Hee Tae; Ha, Su; Dordick, Jonathan S.; Kim, Jungbae.

In: Scientific Reports, Vol. 7, 40202, 05.01.2017.

Research output: Contribution to journalArticle

Kim, Byoung Chan ; Lee, Inseon ; Kwon, Seok Joon ; Wee, Youngho ; Kwon, Ki Young ; Jeon, Chulmin ; An, Hyo Jin ; Jung, Hee Tae ; Ha, Su ; Dordick, Jonathan S. ; Kim, Jungbae. / Fabrication of enzyme-based coatings on intact multi-walled carbon nanotubes as highly effective electrodes in biofuel cells. In: Scientific Reports. 2017 ; Vol. 7.
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