Fabrication of hybrid scaffolds by polymer deposition system and its in-vivo evaluation with a rat tibial defect model

Min Woo Sa, Sung Eun Kim, Young Pil Yun, Hae Ryong Song, Jong Young Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


The purpose of this study was to investigate the bone regeneration ability of a polycaprolactone (PCL) tube scaffold fabricated by using a polymer deposition system with G-code and to evaluate the biocompatibility of bone graft material with Bio-C (HA (30%)/TCP (70%)), carboxymethyl cellulose (CMC), and bone morphogenetic protein-2(BMP-2). The fabrication of a rapid prototyping-based PCL tube scaffold requires a combination of several devices, including a heater, pressure dispenser, and motion controller, etc. This system can process polymer with high precision by a 200-μm nozzle. We used scanning electron microscopy to observe the surface of fabricated scaffold. Three groups considered in this study were PCL tube scaffold (Group A), BMP-2(0.1 mg)/Bio-C/CMC/PCL scaffold (Group B), and BMP-2(0.5 mg)/Bio-C/CMC/PCL scaffold (Group C). The functional recovery and bone regeneration potential were estimated by performing an in-vivo animal experiment with a white rat model. Then, the effect of the scaffold on tibial defects in rats was examined by observing an X-ray image at 4 or 8 weeks and by carrying out histological analysis. In this study, scaffolds fabricated by using the PDS (polymer deposition system), had a diameter of 4.0 mm and a height of 8.0 mm. Moreover, we confirmed that group C exhibited better biomedical characteristics for bone formation than the other scaffolds. The evaluation of in-vivo experimental results suggested that the co-fabrication of the PCL tube scaffold with group C resulted in sustained bone regeneration, which in turn improved the biocompatibility of the bone graft material.

Original languageEnglish
Pages (from-to)439-445
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Issue number6
Publication statusPublished - 2014 Dec 10


  • Bio-C(composite (HA(30%)/β-TCP(70%))
  • Bone morphogenetic protein-2 (BMP-2)
  • Carboxymethyl cellulose (CMC)
  • Polycaprolactone (PCL)
  • Polymer deposition system (PDS)
  • Scaffold

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering


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