Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion

P. Kim, D. H. Kim, B. Kim, S. K. Choi, S. H. Lee, A. Khademhosseini, R. Langer, K. Y. Suh

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

A simple method was developed to fabricate polyethylene glycol (PEG) nanostructures using capillary lithography mediated by ultraviolet (UV) exposure. Acrylate-containing PEG monomers, such as PEG dimethacrylate (PEG-DMA, MW ≤ 330), were photo-cross-linked under UV exposure to generate patterned structures. In comparison to unpatterned PEG films, hydrophobicity of PEG nanostructure modified surfaces was significantly enhanced. This could be attributed to trapped air in the nanostructures as supported by water contact angle measurements. Proteins (fibronectin, immunoglobulin, and albumin) and cells (fibroblasts and P19 EC cells) were examined on the modified surfaces to test for the level of protein adsorption and cell adhesion. It was found that proteins and cells preferred to adhere on nanostructured PEG surfaces in comparison to unpatterned PEG films; however, this level of adhesion was significantly lower than that of glass controls. These results suggest that capillary lithography can be used to fabricate PEG nanostructures capable of modifying protein and cell adhesive properties of surfaces.

Original languageEnglish
Pages (from-to)2420-2426
Number of pages7
JournalNanotechnology
Volume16
Issue number10
DOIs
Publication statusPublished - 2005 Oct 1

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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    Kim, P., Kim, D. H., Kim, B., Choi, S. K., Lee, S. H., Khademhosseini, A., Langer, R., & Suh, K. Y. (2005). Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion. Nanotechnology, 16(10), 2420-2426. https://doi.org/10.1088/0957-4484/16/10/072