Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion

P. Kim, D. H. Kim, B. Kim, S. K. Choi, Sang Ho Lee, A. Khademhosseini, R. Langer, K. Y. Suh

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

A simple method was developed to fabricate polyethylene glycol (PEG) nanostructures using capillary lithography mediated by ultraviolet (UV) exposure. Acrylate-containing PEG monomers, such as PEG dimethacrylate (PEG-DMA, MW ≤ 330), were photo-cross-linked under UV exposure to generate patterned structures. In comparison to unpatterned PEG films, hydrophobicity of PEG nanostructure modified surfaces was significantly enhanced. This could be attributed to trapped air in the nanostructures as supported by water contact angle measurements. Proteins (fibronectin, immunoglobulin, and albumin) and cells (fibroblasts and P19 EC cells) were examined on the modified surfaces to test for the level of protein adsorption and cell adhesion. It was found that proteins and cells preferred to adhere on nanostructured PEG surfaces in comparison to unpatterned PEG films; however, this level of adhesion was significantly lower than that of glass controls. These results suggest that capillary lithography can be used to fabricate PEG nanostructures capable of modifying protein and cell adhesive properties of surfaces.

Original languageEnglish
Pages (from-to)2420-2426
Number of pages7
JournalNanotechnology
Volume16
Issue number10
DOIs
Publication statusPublished - 2005 Oct 1

Fingerprint

Nanostructures
Cell adhesion
Cell Adhesion
Polyethylene glycols
Adsorption
glycols
polyethylenes
adhesion
proteins
Proteins
Fabrication
fabrication
adsorption
cells
Lithography
lithography
Surface Properties
fibroblasts
Dynamic mechanical analysis
Fibroblasts

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Materials Science(all)
  • Physics and Astronomy (miscellaneous)

Cite this

Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion. / Kim, P.; Kim, D. H.; Kim, B.; Choi, S. K.; Lee, Sang Ho; Khademhosseini, A.; Langer, R.; Suh, K. Y.

In: Nanotechnology, Vol. 16, No. 10, 01.10.2005, p. 2420-2426.

Research output: Contribution to journalArticle

Kim, P, Kim, DH, Kim, B, Choi, SK, Lee, SH, Khademhosseini, A, Langer, R & Suh, KY 2005, 'Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion', Nanotechnology, vol. 16, no. 10, pp. 2420-2426. https://doi.org/10.1088/0957-4484/16/10/072
Kim, P. ; Kim, D. H. ; Kim, B. ; Choi, S. K. ; Lee, Sang Ho ; Khademhosseini, A. ; Langer, R. ; Suh, K. Y. / Fabrication of nanostructures of polyethylene glycol for applications to protein adsorption and cell adhesion. In: Nanotechnology. 2005 ; Vol. 16, No. 10. pp. 2420-2426.
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