Fabrication of the PDMS microchip for serially diluting sample with buffer

J. K. Chang, H. Bang, S. J. Park, Seok Chung, C. Chung, D. C. Han

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We developed a polymer polydimethylsiloxane (PDMS) based microfluidic device that dilutes biological samples with buffer solutions with serially increasing diluted sample concentrations. The device showed the relatively high accuracy in terms of dilution ratios along with the fact that it was faster and easier to operate. With two simple disposable plastic syringes, the plastic microfluidic chip completes ordinary sample dilution sequences faster and more precisely than the conventional manual pipette process in biological or chemical laboratory. The serially diluting mechanism of the microchip is simply that the number of microchannels with the same flow rate determines the total amount of flow into the wells. The soft lithography fabricated the microchannels of tetragonal section length of 50 μm of each side of the microfluidic chip.

Original languageEnglish
Pages (from-to)555-558
Number of pages4
JournalMicrosystem Technologies
Volume9
Issue number8
DOIs
Publication statusPublished - 2003 Oct 1
Externally publishedYes

Fingerprint

Polydimethylsiloxane
Microfluidics
Buffers
buffers
microchannels
Microchannels
Fabrication
Dilution
fabrication
dilution
plastics
Chemical laboratories
chips
pipettes
Plastics
syringes
Syringes
microfluidic devices
Lithography
Polymers

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Instrumentation

Cite this

Fabrication of the PDMS microchip for serially diluting sample with buffer. / Chang, J. K.; Bang, H.; Park, S. J.; Chung, Seok; Chung, C.; Han, D. C.

In: Microsystem Technologies, Vol. 9, No. 8, 01.10.2003, p. 555-558.

Research output: Contribution to journalArticle

Chang, J. K. ; Bang, H. ; Park, S. J. ; Chung, Seok ; Chung, C. ; Han, D. C. / Fabrication of the PDMS microchip for serially diluting sample with buffer. In: Microsystem Technologies. 2003 ; Vol. 9, No. 8. pp. 555-558.
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