Facultative parthenogenesis in the burrowing mayfly, Ephoron eophilum (Ephemeroptera: Polymitarcyidae) with an extremely short alate stage

Kazuki Sekiné, Koji Tojo, Yeon Jae Bae

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Facultative parthenogenesis is important for mayflies with short alate stages because females are able to reproduce without mating. We studied facultative parthenogenesis in Ephoron eophilum, a mayfly with an extremely short alate stage. We examined the survival rates of embryos from unfertilized eggs, in addition to investigating the number of chromosomes in parthenogenetic offspring and the mode of inheritance by nuclear genetic analyses using Exon-Primed Intron-Crossing markers. The survival rate of thelytokous embryos was 0-70.2% (16.7 ± 26.7%, mean ± S.D.). Sixteen chromosomes were present throughout most of the mitotic metaphase in parthenogenetic offspring, which was similar to the number recorded in diploid females. All parthenogenetic offspring were homozygous in nuclear genetic analyses, despite the presence of heterozygous mothers. These results indicate that E. eophilum has the ability to reproduce via facultative parthenogenesis, producing mostly diploid thelytokous offspring. The restoration of ploidy level occurs by automixis via terminal fusion or gamete duplication, and causes rapid reduction of heterozygosity. However, despite this, significant deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the studied populations. This is because facultative parthenogenesis in these circumstances normally has little influence on population genetic structuring, even though parthenogenetic embryos exhibit a high survival rate. The lack of influence of parthenogenesis on the population structure of the natural population strongly suggests that parthenogenesis rarely occurs under natural circumstances.

Original languageEnglish
Pages (from-to)606-612
Number of pages7
JournalEuropean Journal of Entomology
Volume112
Issue number4
DOIs
Publication statusPublished - 2015

Fingerprint

Ephoron
Polymitarcyidae
parthenogenesis
Ephemeroptera
burrowing
embryo (animal)
survival rate
diploidy
chromosomes
ploidy
metaphase
exons
introns
inheritance (genetics)
population genetics
germ cells
heterozygosity
population structure

Keywords

  • Diploid thelytoky
  • Ephemeroptera
  • Ephoron eophilum
  • Exon-primed intron-crossing (EPIC) markers
  • Facultative parthenogenesis
  • Hardy-Weinberg equilibrium
  • Short adult stage

ASJC Scopus subject areas

  • Insect Science

Cite this

Facultative parthenogenesis in the burrowing mayfly, Ephoron eophilum (Ephemeroptera : Polymitarcyidae) with an extremely short alate stage. / Sekiné, Kazuki; Tojo, Koji; Bae, Yeon Jae.

In: European Journal of Entomology, Vol. 112, No. 4, 2015, p. 606-612.

Research output: Contribution to journalArticle

@article{b1af2953927442bf962039e265858266,
title = "Facultative parthenogenesis in the burrowing mayfly, Ephoron eophilum (Ephemeroptera: Polymitarcyidae) with an extremely short alate stage",
abstract = "Facultative parthenogenesis is important for mayflies with short alate stages because females are able to reproduce without mating. We studied facultative parthenogenesis in Ephoron eophilum, a mayfly with an extremely short alate stage. We examined the survival rates of embryos from unfertilized eggs, in addition to investigating the number of chromosomes in parthenogenetic offspring and the mode of inheritance by nuclear genetic analyses using Exon-Primed Intron-Crossing markers. The survival rate of thelytokous embryos was 0-70.2{\%} (16.7 ± 26.7{\%}, mean ± S.D.). Sixteen chromosomes were present throughout most of the mitotic metaphase in parthenogenetic offspring, which was similar to the number recorded in diploid females. All parthenogenetic offspring were homozygous in nuclear genetic analyses, despite the presence of heterozygous mothers. These results indicate that E. eophilum has the ability to reproduce via facultative parthenogenesis, producing mostly diploid thelytokous offspring. The restoration of ploidy level occurs by automixis via terminal fusion or gamete duplication, and causes rapid reduction of heterozygosity. However, despite this, significant deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the studied populations. This is because facultative parthenogenesis in these circumstances normally has little influence on population genetic structuring, even though parthenogenetic embryos exhibit a high survival rate. The lack of influence of parthenogenesis on the population structure of the natural population strongly suggests that parthenogenesis rarely occurs under natural circumstances.",
keywords = "Diploid thelytoky, Ephemeroptera, Ephoron eophilum, Exon-primed intron-crossing (EPIC) markers, Facultative parthenogenesis, Hardy-Weinberg equilibrium, Short adult stage",
author = "Kazuki Sekin{\'e} and Koji Tojo and Bae, {Yeon Jae}",
year = "2015",
doi = "10.14411/eje.2015.074",
language = "English",
volume = "112",
pages = "606--612",
journal = "European Journal of Entomology",
issn = "1210-5759",
publisher = "Czech Academy of Sciences",
number = "4",

}

TY - JOUR

T1 - Facultative parthenogenesis in the burrowing mayfly, Ephoron eophilum (Ephemeroptera

T2 - Polymitarcyidae) with an extremely short alate stage

AU - Sekiné, Kazuki

AU - Tojo, Koji

AU - Bae, Yeon Jae

PY - 2015

Y1 - 2015

N2 - Facultative parthenogenesis is important for mayflies with short alate stages because females are able to reproduce without mating. We studied facultative parthenogenesis in Ephoron eophilum, a mayfly with an extremely short alate stage. We examined the survival rates of embryos from unfertilized eggs, in addition to investigating the number of chromosomes in parthenogenetic offspring and the mode of inheritance by nuclear genetic analyses using Exon-Primed Intron-Crossing markers. The survival rate of thelytokous embryos was 0-70.2% (16.7 ± 26.7%, mean ± S.D.). Sixteen chromosomes were present throughout most of the mitotic metaphase in parthenogenetic offspring, which was similar to the number recorded in diploid females. All parthenogenetic offspring were homozygous in nuclear genetic analyses, despite the presence of heterozygous mothers. These results indicate that E. eophilum has the ability to reproduce via facultative parthenogenesis, producing mostly diploid thelytokous offspring. The restoration of ploidy level occurs by automixis via terminal fusion or gamete duplication, and causes rapid reduction of heterozygosity. However, despite this, significant deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the studied populations. This is because facultative parthenogenesis in these circumstances normally has little influence on population genetic structuring, even though parthenogenetic embryos exhibit a high survival rate. The lack of influence of parthenogenesis on the population structure of the natural population strongly suggests that parthenogenesis rarely occurs under natural circumstances.

AB - Facultative parthenogenesis is important for mayflies with short alate stages because females are able to reproduce without mating. We studied facultative parthenogenesis in Ephoron eophilum, a mayfly with an extremely short alate stage. We examined the survival rates of embryos from unfertilized eggs, in addition to investigating the number of chromosomes in parthenogenetic offspring and the mode of inheritance by nuclear genetic analyses using Exon-Primed Intron-Crossing markers. The survival rate of thelytokous embryos was 0-70.2% (16.7 ± 26.7%, mean ± S.D.). Sixteen chromosomes were present throughout most of the mitotic metaphase in parthenogenetic offspring, which was similar to the number recorded in diploid females. All parthenogenetic offspring were homozygous in nuclear genetic analyses, despite the presence of heterozygous mothers. These results indicate that E. eophilum has the ability to reproduce via facultative parthenogenesis, producing mostly diploid thelytokous offspring. The restoration of ploidy level occurs by automixis via terminal fusion or gamete duplication, and causes rapid reduction of heterozygosity. However, despite this, significant deviation from Hardy-Weinberg equilibrium (HWE) was not observed in the studied populations. This is because facultative parthenogenesis in these circumstances normally has little influence on population genetic structuring, even though parthenogenetic embryos exhibit a high survival rate. The lack of influence of parthenogenesis on the population structure of the natural population strongly suggests that parthenogenesis rarely occurs under natural circumstances.

KW - Diploid thelytoky

KW - Ephemeroptera

KW - Ephoron eophilum

KW - Exon-primed intron-crossing (EPIC) markers

KW - Facultative parthenogenesis

KW - Hardy-Weinberg equilibrium

KW - Short adult stage

UR - http://www.scopus.com/inward/record.url?scp=84950237613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84950237613&partnerID=8YFLogxK

U2 - 10.14411/eje.2015.074

DO - 10.14411/eje.2015.074

M3 - Article

AN - SCOPUS:84950237613

VL - 112

SP - 606

EP - 612

JO - European Journal of Entomology

JF - European Journal of Entomology

SN - 1210-5759

IS - 4

ER -