Fast filtration with a vacuum manifold system as a rapid and robust metabolome sampling method for Saccharomyces cerevisiae

Eun Ju Yun, Seung Oh Seo, Suryang Kwak, Eun Joong Oh, Sun Hee Lee, Yong Su Jin, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

Abstract

For metabolome sample preparation, rapid quenching of cellular metabolism without severe loss of intracellular metabolites is important. Here, we comparatively evaluated the two sampling methods, cold methanol quenching and fast filtration, for one of the most important industrial microorganisms, yeast Saccharomyces cerevisiae. The latter, using a vacuum manifold system capable of processing up to twenty samples simultaneously, was developed here. The results showed that the cold methanol quenching method resulted in severe losses of intracellular metabolites but that sugars and sugar phosphates were highly preserved. In comparison, the fast filtration method better preserved most metabolites with less extracellular contamination. Our results suggested that the fast filtration method with a vacuum manifold system can be widely applied to yeast metabolome analysis due to its simplicity, robustness, and lack of significant intracellular metabolite loss.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalProcess Biochemistry
Volume110
DOIs
Publication statusPublished - 2021 Nov

Keywords

  • Cold methanol quenching
  • Fast filtration
  • Gas chromatography–mass spectrometry
  • Metabolome sample preparation
  • Metabolomics
  • Saccharomyces cerevisiae
  • Sampling method

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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