Fast, three-dimensional super-resolution imaging of live cells

Sara A. Jones, Sang-Hee Shim, Jiang He, Xiaowei Zhuang

Research output: Contribution to journalArticle

483 Citations (Scopus)

Abstract

We report super-resolution fluorescence imaging of live cells with high spatiotemporal resolution using stochastic optical reconstruction microscopy (STORM). By labeling proteins either directly or via SNAP tags with photoswitchable dyes, we obtained two-dimensional (2D) and 3D super-resolution images of living cells, using clathrin-coated pits and the transferrin cargo as model systems. Bright, fast-switching probes enabled us to achieve 2D imaging at spatial resolutions of 1/425 nm and temporal resolutions as fast as 0.5 s. We also demonstrated live-cell 3D super-resolution imaging. We obtained 3D spatial resolution of 1/430 nm in the lateral direction and 1/450 nm in the axial direction at time resolutions as fast as 1-2 s with several independent snapshots. Using photoswitchable dyes with distinct emission wavelengths, we also demonstrated two-color 3D super-resolution imaging in live cells. These imaging capabilities open a new window for characterizing cellular structures in living cells at the ultrastructural level.

Original languageEnglish
Pages (from-to)499-505
Number of pages7
JournalNature Methods
Volume8
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1
Externally publishedYes

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Imaging techniques
Coloring Agents
Cells
Clathrin
Optical Imaging
Cellular Structures
Transferrin
Image resolution
Labeling
Microscopy
Microscopic examination
Color
Fluorescence
Wavelength
Proteins
Direction compound

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fast, three-dimensional super-resolution imaging of live cells. / Jones, Sara A.; Shim, Sang-Hee; He, Jiang; Zhuang, Xiaowei.

In: Nature Methods, Vol. 8, No. 6, 01.06.2011, p. 499-505.

Research output: Contribution to journalArticle

Jones, Sara A. ; Shim, Sang-Hee ; He, Jiang ; Zhuang, Xiaowei. / Fast, three-dimensional super-resolution imaging of live cells. In: Nature Methods. 2011 ; Vol. 8, No. 6. pp. 499-505.
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