Fate of neutrophils during the recovery phase of ischemia/ reperfusion induced acute kidney injury

Wonyong Cho, Jie Young Song, Se Won Oh, Myung Gyu Kim, Yoon Sook Ko, Hee Yong Lee, Sang Kyung Jo

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Effective clearance of inflammatory cells is required for resolution of inflammation. Here, we show in vivo evidence that apoptosis and reverse transendothelial migration (rTEM) are important mechanisms in eliminating neutrophils and facilitating recovery following ischemia/reperfusion injury (IRI) of the kidney. The clearance of neutrophils was delayed in the Bax knockout (KO)BM → wild-type (WT) chimera in which bone marrow derived cells are partially resistant to apoptosis, compared to WTBM → WT mice. These mice also showed delayed functional, histological recovery, increased tissue cytokines, and accelerated fibrosis. The circulating intercellular adhesion molecule-1 (ICAM-1)+ Gr-1+ neutrophils displaying rTEM phenotype increased during the recovery phase and blockade of junctional adhesion molecule-C (JAM-C), a negative regulator of rTEM, resulted in an increase in circulating ICAM-1+ neutrophils, faster resolution of inflammation and recovery. The presence of Tamm-Horsfall protein (THP) in circulating ICAM-1+ neutrophils could suggest that they are derived from injured kidneys. In conclusion, we suggest that apoptosis and rTEM are critically involved in the clearance mechanisms of neutrophils during the recovery phase of IRI.

    Original languageEnglish
    Pages (from-to)1616-1625
    Number of pages10
    JournalJournal of Korean medical science
    Volume32
    Issue number10
    DOIs
    Publication statusPublished - 2017 Oct 1

    Keywords

    • Apoptosis
    • Ischemia/reperfusion
    • Neutrophil
    • Recovery
    • Reverse transendothelial migration

    ASJC Scopus subject areas

    • Medicine(all)

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