Fatty acid profiling and proteomic analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide

Soo Rin Kim, Hee Taek Kim, Hee Jung Park, Sooah Kim, Hee Jung Choi, Geum Sook Hwang, Jong Hoon Yi, Do Hyun Ryu, Kyoung Heon Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. In particular, since supercritical carbon dioxide (SC-CO2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. Although SC-CO2 has been applied to many microorganisms, the inactivation of microbial cells by SC-CO2 has only been evaluated by using a conventional viable cell count such as a plating method, by which it is not possible to systematically elucidate the microbial cell inactivation process. Therefore, in this study the physiological status of SC-CO2 treated Salmonella enterica serotype Typhimurium was analyzed by using GC-MS analysis of fatty acids with principal component analysis and two-dimensional electrophoresis for protein profiling. From the results of these systemic analyses, it was revealed that SC-CO2 caused significant alterations to the profiles of fatty acids and proteins of the cells.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalInternational Journal of Food Microbiology
Volume134
Issue number3
DOIs
Publication statusPublished - 2009 Sep 15

Keywords

  • GC-MS
  • Salmonella enterica serotype Typhimurium
  • Sterilization
  • Supercritical carbon dioxide
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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