Fed-batch cultivation for the production of clavulanic acid by an immobilized Streptomyces clavuligerus mutant

Il Chul Kim, Chang Ho Kim, Suk In Hong, Seung Wook Kim

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In order to obtain high productivity of clavulanic acid, a newly-introduced carrier, polyurethane pellet (PUP) Z97-020 was used for the immobilization process. In a stirred-tank bioreactor, batch cultivation by Streptomyces clavuligerus KK immobilized on PUP Z97-020 gave about 3100 mg of clavulanic acid per litre, representing an increase of 200% in productivity compared with that by fed-batch cultivation of free cells (1500 mg/l). However, the clavulanic acid produced rapidly decomposed due to the pH change during batch cultivation. Fed-batch cultivation by immobilized S. clavuligerus KK gave an excellent level of clavulanic acid up to 3250 mg/l, a productivity increase of 220% compared with that by fed-batch cultivation of free cells. These results suggest that immobilization with PUP Z97-020 is a more effective process for the production of clavulanic acid and that the maintenance of pH by fed-batch cultivation with glycerol as a limiting substrate prevents the clavulanic acid from decomposing during the fermentation.

Original languageEnglish
Pages (from-to)869-872
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Issue number9
Publication statusPublished - 2001 Dec 1



  • Clavulanic acid
  • Fed-batch cultivation
  • Immobilization
  • Streptomyces clavuligerus KK

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Plant Science
  • Biochemistry
  • Food Science
  • Microbiology

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