Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation

Chang Duk Kang, Se Jong Han, Seung Phill Choi, Sang Jun Sim

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume33
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Fingerprint

Photobioreactors
Batch Cell Culture Techniques
Nutrients
Biomass
Productivity
Light
Food
Nitrates
Phosphates
Photons
Lighting
Cells
Growth
astaxanthine

Keywords

  • Astaxanthin
  • Exponential feeding
  • Fed-batch culture
  • Haematococcus pluvialis
  • Photoautotrophic induction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation. / Kang, Chang Duk; Han, Se Jong; Choi, Seung Phill; Sim, Sang Jun.

In: Bioprocess and Biosystems Engineering, Vol. 33, No. 1, 01.01.2010, p. 133-139.

Research output: Contribution to journalArticle

@article{0867261288e94c68a0731fd1ca4a6b50,
title = "Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation",
abstract = "A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.",
keywords = "Astaxanthin, Exponential feeding, Fed-batch culture, Haematococcus pluvialis, Photoautotrophic induction",
author = "Kang, {Chang Duk} and Han, {Se Jong} and Choi, {Seung Phill} and Sim, {Sang Jun}",
year = "2010",
month = "1",
day = "1",
doi = "10.1007/s00449-009-0362-5",
language = "English",
volume = "33",
pages = "133--139",
journal = "Bioprocess and Biosystems Engineering",
issn = "1615-7591",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Fed-batch culture of astaxanthin-rich Haematococcus pluvialis by exponential nutrient feeding and stepwise light supplementation

AU - Kang, Chang Duk

AU - Han, Se Jong

AU - Choi, Seung Phill

AU - Sim, Sang Jun

PY - 2010/1/1

Y1 - 2010/1/1

N2 - A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.

AB - A fed-batch culture process followed by subsequent photoautotrophic induction was established for the high density culture of astaxanthin-rich Haematococcus pluvialis using a CO2-fed flat type photobioreactor under unsynchronized illumination. Fed-batch culture was performed with an exponential feeding strategy of the growth-limiting nutrients, nitrate and phosphate, concurrently with the stepwise supplementation of light depending on the cell concentration. During the growth phase, a biomass of 1.47 g/L was obtained at a biomass productivity of 0.33 g/L/day. Photoautotrophic induction of the well-grown vegetative cells was performed consecutively by increasing the light intensity to 400 μmol photon/m2/s, while keeping the other conditions in the CO2-fed flat type photobioreactor fixed, yielding an astaxanthin production of 190 mg/L at an astaxanthin productivity of 14 mg/L/day. The proposed sequential photoautotrophic process has high potential as simple and productive process for the production of valuable Haematococcus astaxanthin.

KW - Astaxanthin

KW - Exponential feeding

KW - Fed-batch culture

KW - Haematococcus pluvialis

KW - Photoautotrophic induction

UR - http://www.scopus.com/inward/record.url?scp=76849099295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76849099295&partnerID=8YFLogxK

U2 - 10.1007/s00449-009-0362-5

DO - 10.1007/s00449-009-0362-5

M3 - Article

C2 - 19662437

AN - SCOPUS:76849099295

VL - 33

SP - 133

EP - 139

JO - Bioprocess and Biosystems Engineering

JF - Bioprocess and Biosystems Engineering

SN - 1615-7591

IS - 1

ER -