Abstract
To reduce the distillation costs of cellulosic ethanol, it is necessary to produce high sugar titers in the enzymatic saccharification step. To obtain high sugar titers, high biomass loadings of lignocellulose are necessary. In this study, to overcome the low saccharification yields and the low operability of high biomass loadings, a fed-batch saccharification process was developed using an enzyme reactor that was designed and built in-house. After optimizing the cellulase and biomass feeding profiles and the agitation speed, 132.6 g/L glucose and 76.0% theoretical maximum glucose were obtained from the 60 h saccharification of maleic acid-pretreated rice straw at a 30% (w/v) solids loading with 15 filter paper units (FPU) of Cellic CTec2/g glucan. This study demonstrated that through the proper optimization of fed-batch saccharification, both high sugar titers and high saccharification yields are possible, even with using the high solids loading (i.e., ≥30%) with the moderate enzyme loading (i.e., <15 FPU/g glucan). These results could be contributed to improving economic feasibility of the high solids saccharification process in cellulosic fuel and chemical production.
| Original language | English |
|---|---|
| Pages (from-to) | 1-13 |
| Number of pages | 13 |
| Journal | Applied Biochemistry and Biotechnology |
| DOIs | |
| Publication status | Accepted/In press - 2017 Jan 11 |
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Keywords
- Cellulase
- Fed-batch
- High solids loading
- Lignocellulose
- Saccharification
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
Cite this
Fed-Batch Enzymatic Saccharification of High Solids Pretreated Lignocellulose for Obtaining High Titers and High Yields of Glucose. / Jung, Young Hoon; Park, Hyun Min; Kim, Dong Hyun; Yang, Jungwoo; Kim, Kyoung Heon.
In: Applied Biochemistry and Biotechnology, 11.01.2017, p. 1-13.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fed-Batch Enzymatic Saccharification of High Solids Pretreated Lignocellulose for Obtaining High Titers and High Yields of Glucose
AU - Jung, Young Hoon
AU - Park, Hyun Min
AU - Kim, Dong Hyun
AU - Yang, Jungwoo
AU - Kim, Kyoung Heon
PY - 2017/1/11
Y1 - 2017/1/11
N2 - To reduce the distillation costs of cellulosic ethanol, it is necessary to produce high sugar titers in the enzymatic saccharification step. To obtain high sugar titers, high biomass loadings of lignocellulose are necessary. In this study, to overcome the low saccharification yields and the low operability of high biomass loadings, a fed-batch saccharification process was developed using an enzyme reactor that was designed and built in-house. After optimizing the cellulase and biomass feeding profiles and the agitation speed, 132.6 g/L glucose and 76.0% theoretical maximum glucose were obtained from the 60 h saccharification of maleic acid-pretreated rice straw at a 30% (w/v) solids loading with 15 filter paper units (FPU) of Cellic CTec2/g glucan. This study demonstrated that through the proper optimization of fed-batch saccharification, both high sugar titers and high saccharification yields are possible, even with using the high solids loading (i.e., ≥30%) with the moderate enzyme loading (i.e., <15 FPU/g glucan). These results could be contributed to improving economic feasibility of the high solids saccharification process in cellulosic fuel and chemical production.
AB - To reduce the distillation costs of cellulosic ethanol, it is necessary to produce high sugar titers in the enzymatic saccharification step. To obtain high sugar titers, high biomass loadings of lignocellulose are necessary. In this study, to overcome the low saccharification yields and the low operability of high biomass loadings, a fed-batch saccharification process was developed using an enzyme reactor that was designed and built in-house. After optimizing the cellulase and biomass feeding profiles and the agitation speed, 132.6 g/L glucose and 76.0% theoretical maximum glucose were obtained from the 60 h saccharification of maleic acid-pretreated rice straw at a 30% (w/v) solids loading with 15 filter paper units (FPU) of Cellic CTec2/g glucan. This study demonstrated that through the proper optimization of fed-batch saccharification, both high sugar titers and high saccharification yields are possible, even with using the high solids loading (i.e., ≥30%) with the moderate enzyme loading (i.e., <15 FPU/g glucan). These results could be contributed to improving economic feasibility of the high solids saccharification process in cellulosic fuel and chemical production.
KW - Cellulase
KW - Fed-batch
KW - High solids loading
KW - Lignocellulose
KW - Saccharification
UR - http://www.scopus.com/inward/record.url?scp=85009275268&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009275268&partnerID=8YFLogxK
U2 - 10.1007/s12010-016-2385-0
DO - 10.1007/s12010-016-2385-0
M3 - Article
C2 - 28078651
AN - SCOPUS:85009275268
SP - 1
EP - 13
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
SN - 0273-2289
ER -