Fed-Batch Enzymatic Saccharification of High Solids Pretreated Lignocellulose for Obtaining High Titers and High Yields of Glucose

Young Hoon Jung, Hyun Min Park, Dong Hyun Kim, Jungwoo Yang, Kyoung Heon Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To reduce the distillation costs of cellulosic ethanol, it is necessary to produce high sugar titers in the enzymatic saccharification step. To obtain high sugar titers, high biomass loadings of lignocellulose are necessary. In this study, to overcome the low saccharification yields and the low operability of high biomass loadings, a fed-batch saccharification process was developed using an enzyme reactor that was designed and built in-house. After optimizing the cellulase and biomass feeding profiles and the agitation speed, 132.6 g/L glucose and 76.0% theoretical maximum glucose were obtained from the 60 h saccharification of maleic acid-pretreated rice straw at a 30% (w/v) solids loading with 15 filter paper units (FPU) of Cellic CTec2/g glucan. This study demonstrated that through the proper optimization of fed-batch saccharification, both high sugar titers and high saccharification yields are possible, even with using the high solids loading (i.e., ≥30%) with the moderate enzyme loading (i.e., <15 FPU/g glucan). These results could be contributed to improving economic feasibility of the high solids saccharification process in cellulosic fuel and chemical production.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalApplied Biochemistry and Biotechnology
DOIs
Publication statusAccepted/In press - 2017 Jan 11

Keywords

  • Cellulase
  • Fed-batch
  • High solids loading
  • Lignocellulose
  • Saccharification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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