Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1

Jong Hwan Shin, Jong Hyun Yoon, Kyoung Ahn Eun Kyoung Ahn, Mi S. Kim, Sang Jun Sim, Tai Hyun Park

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA-DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4-7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol.

Original languageEnglish
Pages (from-to)192-199
Number of pages8
JournalInternational Journal of Hydrogen Energy
Volume32
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1
Externally publishedYes

Fingerprint

hydrogen production
Hydrogen production
Hydrogen
formic acid
hydrogen
glucose
Glucose
bacteria
Bacteria
formates
DNA
deoxyribonucleic acid
landfills
Fructose
fermentation
sequencing
Sorbitol
sucrose
Sugar (sucrose)
Land fill

Keywords

  • Enterobacter asburiae SNU-1
  • Fermentation
  • Formate decomposition
  • Hydrogen production

ASJC Scopus subject areas

  • Electrochemistry
  • Fuel Technology
  • Renewable Energy, Sustainability and the Environment

Cite this

Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1. / Shin, Jong Hwan; Hyun Yoon, Jong; Eun Kyoung Ahn, Kyoung Ahn; Kim, Mi S.; Sim, Sang Jun; Park, Tai Hyun.

In: International Journal of Hydrogen Energy, Vol. 32, No. 2, 01.02.2007, p. 192-199.

Research output: Contribution to journalArticle

Shin, Jong Hwan ; Hyun Yoon, Jong ; Eun Kyoung Ahn, Kyoung Ahn ; Kim, Mi S. ; Sim, Sang Jun ; Park, Tai Hyun. / Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1. In: International Journal of Hydrogen Energy. 2007 ; Vol. 32, No. 2. pp. 192-199.
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