Abstract
A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA-DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4-7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol.
| Original language | English |
|---|---|
| Pages (from-to) | 192-199 |
| Number of pages | 8 |
| Journal | International Journal of Hydrogen Energy |
| Volume | 32 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2007 Feb 1 |
| Externally published | Yes |
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Keywords
- Enterobacter asburiae SNU-1
- Fermentation
- Formate decomposition
- Hydrogen production
ASJC Scopus subject areas
- Electrochemistry
- Fuel Technology
- Renewable Energy, Sustainability and the Environment
Cite this
Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1. / Shin, Jong Hwan; Hyun Yoon, Jong; Eun Kyoung Ahn, Kyoung Ahn; Kim, Mi S.; Sim, Sang Jun; Park, Tai Hyun.
In: International Journal of Hydrogen Energy, Vol. 32, No. 2, 01.02.2007, p. 192-199.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1
AU - Shin, Jong Hwan
AU - Hyun Yoon, Jong
AU - Eun Kyoung Ahn, Kyoung Ahn
AU - Kim, Mi S.
AU - Sim, Sang Jun
AU - Park, Tai Hyun
PY - 2007/2/1
Y1 - 2007/2/1
N2 - A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA-DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4-7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol.
AB - A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA-DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4-7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol.
KW - Enterobacter asburiae SNU-1
KW - Fermentation
KW - Formate decomposition
KW - Hydrogen production
UR - http://www.scopus.com/inward/record.url?scp=33846237251&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846237251&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2006.08.013
DO - 10.1016/j.ijhydene.2006.08.013
M3 - Article
AN - SCOPUS:33846237251
VL - 32
SP - 192
EP - 199
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
SN - 0360-3199
IS - 2
ER -