Fermented soy bean extract suppresses differentiation of 3T3-L1 preadipocytes and facilitates its glucose utilization

Ji Won Hwang, Hyun Ju Do, Oh Yoen Kim, Ji Hyung Chung, Jae Yeon Lee, Young Shik Park, Kyo Yeol Hwang, Su Il Seong, Min-Jeong Shin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Obesity is a serious health problem which may continuously increase the morbidity and mortality associated with a variety of acute and chronic diseases.This study aimed to examine the antiobesity effect of soy bean extract fermented by Bacillus subtilis MORI (BTD-1) and to elucidate the mechanisms underlying such effects using 3T3-L1 preadipocytes. Lipid accumulations were significantly inhibited by BTD-1 treatment, which were accompanied by the decreased expression of CCAAT element binding protein α (C/EBPα) and the increased phosphorylation of acetyl-CoA carboxylase (ACC) protein expression. In addition, BTD-1 treatment increased the expression of glucose transporter 4 (GLUT4) along with significantly increasing glucose uptake into the adipocytes based on results of a 2-deoxy-D-[<sup>3</sup>H] glucose uptake assay. Our findings suggest that BTD-1 may suppress the differentiation of 3T3-L1 adipocytes and greatly facilitate glucose uptake into the adipocytes. Therefore, BTD-1 has a potential as a functional food ingredient in improving obesity and related metabolic disorders.

Original languageEnglish
Pages (from-to)516-524
Number of pages9
JournalJournal of Functional Foods
Volume15
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

adipocytes
Soybeans
Adipocytes
soybeans
uptake mechanisms
Glucose
glucose
extracts
obesity
Obesity
Acetyl-CoA Carboxylase
acute course
acetyl-CoA carboxylase
glucose transporters
Functional Food
Facilitative Glucose Transport Proteins
Deoxyglucose
metabolic diseases
Acute Disease
Bacillus subtilis

Keywords

  • ACC
  • Adipogenesis
  • C/EBPα
  • GLUT4
  • Soy bean extract

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Fermented soy bean extract suppresses differentiation of 3T3-L1 preadipocytes and facilitates its glucose utilization. / Hwang, Ji Won; Do, Hyun Ju; Kim, Oh Yoen; Chung, Ji Hyung; Lee, Jae Yeon; Park, Young Shik; Hwang, Kyo Yeol; Seong, Su Il; Shin, Min-Jeong.

In: Journal of Functional Foods, Vol. 15, 01.05.2015, p. 516-524.

Research output: Contribution to journalArticle

Hwang, Ji Won ; Do, Hyun Ju ; Kim, Oh Yoen ; Chung, Ji Hyung ; Lee, Jae Yeon ; Park, Young Shik ; Hwang, Kyo Yeol ; Seong, Su Il ; Shin, Min-Jeong. / Fermented soy bean extract suppresses differentiation of 3T3-L1 preadipocytes and facilitates its glucose utilization. In: Journal of Functional Foods. 2015 ; Vol. 15. pp. 516-524.
@article{226f5bc925a8428f98e48824182d65d0,
title = "Fermented soy bean extract suppresses differentiation of 3T3-L1 preadipocytes and facilitates its glucose utilization",
abstract = "Obesity is a serious health problem which may continuously increase the morbidity and mortality associated with a variety of acute and chronic diseases.This study aimed to examine the antiobesity effect of soy bean extract fermented by Bacillus subtilis MORI (BTD-1) and to elucidate the mechanisms underlying such effects using 3T3-L1 preadipocytes. Lipid accumulations were significantly inhibited by BTD-1 treatment, which were accompanied by the decreased expression of CCAAT element binding protein α (C/EBPα) and the increased phosphorylation of acetyl-CoA carboxylase (ACC) protein expression. In addition, BTD-1 treatment increased the expression of glucose transporter 4 (GLUT4) along with significantly increasing glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings suggest that BTD-1 may suppress the differentiation of 3T3-L1 adipocytes and greatly facilitate glucose uptake into the adipocytes. Therefore, BTD-1 has a potential as a functional food ingredient in improving obesity and related metabolic disorders.",
keywords = "ACC, Adipogenesis, C/EBPα, GLUT4, Soy bean extract",
author = "Hwang, {Ji Won} and Do, {Hyun Ju} and Kim, {Oh Yoen} and Chung, {Ji Hyung} and Lee, {Jae Yeon} and Park, {Young Shik} and Hwang, {Kyo Yeol} and Seong, {Su Il} and Min-Jeong Shin",
year = "2015",
month = "5",
day = "1",
doi = "10.1016/j.jff.2015.04.002",
language = "English",
volume = "15",
pages = "516--524",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Fermented soy bean extract suppresses differentiation of 3T3-L1 preadipocytes and facilitates its glucose utilization

AU - Hwang, Ji Won

AU - Do, Hyun Ju

AU - Kim, Oh Yoen

AU - Chung, Ji Hyung

AU - Lee, Jae Yeon

AU - Park, Young Shik

AU - Hwang, Kyo Yeol

AU - Seong, Su Il

AU - Shin, Min-Jeong

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Obesity is a serious health problem which may continuously increase the morbidity and mortality associated with a variety of acute and chronic diseases.This study aimed to examine the antiobesity effect of soy bean extract fermented by Bacillus subtilis MORI (BTD-1) and to elucidate the mechanisms underlying such effects using 3T3-L1 preadipocytes. Lipid accumulations were significantly inhibited by BTD-1 treatment, which were accompanied by the decreased expression of CCAAT element binding protein α (C/EBPα) and the increased phosphorylation of acetyl-CoA carboxylase (ACC) protein expression. In addition, BTD-1 treatment increased the expression of glucose transporter 4 (GLUT4) along with significantly increasing glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings suggest that BTD-1 may suppress the differentiation of 3T3-L1 adipocytes and greatly facilitate glucose uptake into the adipocytes. Therefore, BTD-1 has a potential as a functional food ingredient in improving obesity and related metabolic disorders.

AB - Obesity is a serious health problem which may continuously increase the morbidity and mortality associated with a variety of acute and chronic diseases.This study aimed to examine the antiobesity effect of soy bean extract fermented by Bacillus subtilis MORI (BTD-1) and to elucidate the mechanisms underlying such effects using 3T3-L1 preadipocytes. Lipid accumulations were significantly inhibited by BTD-1 treatment, which were accompanied by the decreased expression of CCAAT element binding protein α (C/EBPα) and the increased phosphorylation of acetyl-CoA carboxylase (ACC) protein expression. In addition, BTD-1 treatment increased the expression of glucose transporter 4 (GLUT4) along with significantly increasing glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings suggest that BTD-1 may suppress the differentiation of 3T3-L1 adipocytes and greatly facilitate glucose uptake into the adipocytes. Therefore, BTD-1 has a potential as a functional food ingredient in improving obesity and related metabolic disorders.

KW - ACC

KW - Adipogenesis

KW - C/EBPα

KW - GLUT4

KW - Soy bean extract

UR - http://www.scopus.com/inward/record.url?scp=84929241127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929241127&partnerID=8YFLogxK

U2 - 10.1016/j.jff.2015.04.002

DO - 10.1016/j.jff.2015.04.002

M3 - Article

VL - 15

SP - 516

EP - 524

JO - Journal of Functional Foods

JF - Journal of Functional Foods

SN - 1756-4646

ER -