Fermented soy bean extract suppresses differentiation of 3T3-L1 preadipocytes and facilitates its glucose utilization

Ji Won Hwang, Hyun Ju Do, Oh Yoen Kim, Ji Hyung Chung, Jae Yeon Lee, Young Shik Park, Kyo Yeol Hwang, Su Il Seong, Min-Jeong Shin

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15 Citations (Scopus)


Obesity is a serious health problem which may continuously increase the morbidity and mortality associated with a variety of acute and chronic diseases.This study aimed to examine the antiobesity effect of soy bean extract fermented by Bacillus subtilis MORI (BTD-1) and to elucidate the mechanisms underlying such effects using 3T3-L1 preadipocytes. Lipid accumulations were significantly inhibited by BTD-1 treatment, which were accompanied by the decreased expression of CCAAT element binding protein α (C/EBPα) and the increased phosphorylation of acetyl-CoA carboxylase (ACC) protein expression. In addition, BTD-1 treatment increased the expression of glucose transporter 4 (GLUT4) along with significantly increasing glucose uptake into the adipocytes based on results of a 2-deoxy-D-[<sup>3</sup>H] glucose uptake assay. Our findings suggest that BTD-1 may suppress the differentiation of 3T3-L1 adipocytes and greatly facilitate glucose uptake into the adipocytes. Therefore, BTD-1 has a potential as a functional food ingredient in improving obesity and related metabolic disorders.

Original languageEnglish
Pages (from-to)516-524
Number of pages9
JournalJournal of Functional Foods
Publication statusPublished - 2015 May 1



  • ACC
  • Adipogenesis
  • C/EBPα
  • GLUT4
  • Soy bean extract

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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