Ferredoxin-NADP + reductase from Pseudomonas putida functions as a ferric reductase

Jinki Yeom, Che Ok Jeon, Eugene L. Madsen, Woojun Park

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Pseudomonas putida harbors two ferredoxin-NADP + reductases (Fprs) on its chromosome, and their functions remain largely unknown. Ferric reductase is structurally contained within the Fpr superfamily. Interestingly, ferric reductase is not annotated on the chromosome of P. putida. In an effort to elucidate the function of the Fpr as a ferric reductase, we used a variety of biochemical and physiological methods using the wild-type and mutant strains. In both the ferric reductase and flavin reductase assays, FprA and FprB preferentially used NADPH and NADH as electron donors, respectively. Two Fprs prefer a native ferric chelator to a synthetic ferric chelator and utilize free flavin mononucleotide (FMN) as an electron carrier. FprB has a higher k cat/K m value for reducing the ferric complex with free FMN. The growth rate of the fprB mutant was reduced more profoundly than that of the fprA mutant, the growth rate of which is also lower than the wild type in ferric iron-containing minimal media. Flavin reductase activity was diminished completely when the cell extracts of the fprB mutant plus NADH were utilized, but not the fprA mutant with NADPH. This indicates that other NADPH-dependent flavin reductases may exist. Interestingly, the structure of the NAD(P) region of FprB, but not of FprA, resembled the ferric reductase (Fre) of Escherichia coli in the homology modeling. This study demonstrates, for the first time, the functions of Fprs in P. putida as flavin and ferric reductases. Furthermore, our results indicated that FprB may perform a crucial role as a NADH-dependent ferric/flavin reductase under iron stress conditions.

Original languageEnglish
Pages (from-to)1472-1479
Number of pages8
JournalJournal of Bacteriology
Volume191
Issue number5
DOIs
Publication statusPublished - 2009 Mar 1

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Ferredoxin-NADP Reductase
Pseudomonas putida
NAD
Flavin Mononucleotide
Chelating Agents
NADP
FMN Reductase
Oxidoreductases
Iron
Chromosomes
Electrons
Growth
ferric citrate iron reductase
Cell Extracts
Cats
Escherichia coli
4,6-dinitro-o-cresol

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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Ferredoxin-NADP + reductase from Pseudomonas putida functions as a ferric reductase. / Yeom, Jinki; Jeon, Che Ok; Madsen, Eugene L.; Park, Woojun.

In: Journal of Bacteriology, Vol. 191, No. 5, 01.03.2009, p. 1472-1479.

Research output: Contribution to journalArticle

Yeom, Jinki ; Jeon, Che Ok ; Madsen, Eugene L. ; Park, Woojun. / Ferredoxin-NADP + reductase from Pseudomonas putida functions as a ferric reductase. In: Journal of Bacteriology. 2009 ; Vol. 191, No. 5. pp. 1472-1479.
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