Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae

Youngwoo Kim, Cheol-Won Yun, Caroline C. Philpott

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Siderophores are small iron-binding molecules that are synthesized and secreted in the iron-free form by microorganisms. Saccharomyces cerevisiae takes up iron bound to siderophores by two separate systems, one of which requires the ARN family of siderophore-iron transporters. Arn1p and Arn3p are expressed in endosome-like intracellular vesicles. Here we present evidence that, in the absence of its specific substrate, ferrichrome, Arn1p is sorted directly from the Golgi to the endosomal compartment and does not cycle to the plasma membrane. When cells are exposed to ferrichrome at low concentrations, Arn1p stably relocalizes to the plasma membrane. At higher concentrations of ferrichrome, Arn1p relocalizes to the plasma membrane and rapidly undergoes endocytosis. Plasma membrane localization of Arn1p occurs only in the presence of its specific substrate, and not in the presence of other siderophores. Despite expression of Arn1p on the plasma membrane, mutant strains with defects in endocytosis exhibit reduced uptake of ferrichrome-iron. Thus, siderophores influence the trafficking of the Arn transporters within the cell and this trafficking is important for transporter function.

Original languageEnglish
Pages (from-to)3632-3642
Number of pages11
JournalEMBO Journal
Volume21
Issue number14
DOIs
Publication statusPublished - 2002 Jul 15
Externally publishedYes

Fingerprint

Ferrichrome
Siderophores
Endosomes
Cell membranes
Yeast
Saccharomyces cerevisiae
Iron
Cell Membrane
Endocytosis
Substrates
Microorganisms
Defects
Molecules

Keywords

  • Endocytosis
  • Exocytosis
  • Iron
  • Siderophore
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Ferrichrome induces endosome to plasma membrane cycling of the ferrichrome transporter, Arn1p, in Saccharomyces cerevisiae. / Kim, Youngwoo; Yun, Cheol-Won; Philpott, Caroline C.

In: EMBO Journal, Vol. 21, No. 14, 15.07.2002, p. 3632-3642.

Research output: Contribution to journalArticle

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AU - Yun, Cheol-Won

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