FGF2 stimulates osteogenic differentiation through ERK induced TAZ expression

Mi Ran Byun, A. Rum Kim, Jun Ha Hwang, Kyung Min Kim, Eun Sook Hwang, Jeong-Ho Hong

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

TAZ (transcriptional coactivator with PDZ-binding motif) is a transcriptional modulator that regulates mesenchymal stem cell differentiation. It stimulates osteogenic differentiation while inhibiting adipocyte differentiation. FGFs (fibroblast growth factors) stimulate several signaling proteins to regulate their target genes, which are involved in cell proliferation, differentiation, and cell survival. Within this family, FGF2 stimulates osteoblast differentiation though a mechanism that is largely unknown. In this report, we show that TAZ mediates FGF2 signaling in osteogenesis. We observed that FGF2 increases TAZ expression by stimulating its mRNA expression. Depletion of TAZ using small hairpin RNA blocked FGF2-mediated osteogenic differentiation. FGF2 induced TAZ expression was stimulated by ERK (extracellular signal-regulated kinase) activation and the inhibition of ERK blocked TAZ expression. FGF2 increased nuclear localization of TAZ and, thus, facilitated the interaction of TAZ and Runx2, activating Runx2-mediated gene transcription. Taken together, these results suggest that TAZ is an important mediator of FGF2 signaling in osteoblast differentiation.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalBone
Volume58
DOIs
Publication statusPublished - 2014 Jan 1

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Extracellular Signal-Regulated MAP Kinases
Fibroblast Growth Factor 2
Osteoblasts
Cell Differentiation
Fibroblast Growth Factors
Mesenchymal Stromal Cells
Adipocytes
Osteogenesis
Small Interfering RNA
Genes
Cell Survival
Cell Proliferation
Messenger RNA
Proteins

Keywords

  • FGF2
  • Osteogenesis
  • Runx2
  • TAZ

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

FGF2 stimulates osteogenic differentiation through ERK induced TAZ expression. / Byun, Mi Ran; Kim, A. Rum; Hwang, Jun Ha; Kim, Kyung Min; Hwang, Eun Sook; Hong, Jeong-Ho.

In: Bone, Vol. 58, 01.01.2014, p. 72-80.

Research output: Contribution to journalArticle

Byun, Mi Ran ; Kim, A. Rum ; Hwang, Jun Ha ; Kim, Kyung Min ; Hwang, Eun Sook ; Hong, Jeong-Ho. / FGF2 stimulates osteogenic differentiation through ERK induced TAZ expression. In: Bone. 2014 ; Vol. 58. pp. 72-80.
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