Field-based angle-resolved light-scattering study of single live cells

Wonshik Choi, Chung Chieh Yu, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We perform field-based angle-resolved light-scattering measurements from single live cells. We use a laser interferometer to acquire phase and amplitude images of cells at the image plane. The angular scattering spectrum is calculated from the Fourier transform of the field transmitted through the cells. A concurrent 3D refractive index distribution of the same cells is measured using tomographic phase microscopy. By measuring transient increases in light scattering by single cells during exposure to acetic acid, we correlate the scattering properties of single cells with their refractive index distributions and show that results are in good agreement with a model based on the Born approximation.

Original languageEnglish
Pages (from-to)1596-1598
Number of pages3
JournalOptics Letters
Volume33
Issue number14
DOIs
Publication statusPublished - 2008 Jul 15
Externally publishedYes

Fingerprint

light scattering
Light
cells
Refractometry
refractivity
Fourier Analysis
scattering
acetic acid
Born approximation
Acetic Acid
Microscopy
Lasers
interferometers
microscopy
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Choi, W., Yu, C. C., Fang-Yen, C., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2008). Field-based angle-resolved light-scattering study of single live cells. Optics Letters, 33(14), 1596-1598. https://doi.org/10.1364/OL.33.001596

Field-based angle-resolved light-scattering study of single live cells. / Choi, Wonshik; Yu, Chung Chieh; Fang-Yen, Christopher; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.

In: Optics Letters, Vol. 33, No. 14, 15.07.2008, p. 1596-1598.

Research output: Contribution to journalArticle

Choi, W, Yu, CC, Fang-Yen, C, Badizadegan, K, Dasari, RR & Feld, MS 2008, 'Field-based angle-resolved light-scattering study of single live cells', Optics Letters, vol. 33, no. 14, pp. 1596-1598. https://doi.org/10.1364/OL.33.001596
Choi W, Yu CC, Fang-Yen C, Badizadegan K, Dasari RR, Feld MS. Field-based angle-resolved light-scattering study of single live cells. Optics Letters. 2008 Jul 15;33(14):1596-1598. https://doi.org/10.1364/OL.33.001596
Choi, Wonshik ; Yu, Chung Chieh ; Fang-Yen, Christopher ; Badizadegan, Kamran ; Dasari, Ramachandra R. ; Feld, Michael S. / Field-based angle-resolved light-scattering study of single live cells. In: Optics Letters. 2008 ; Vol. 33, No. 14. pp. 1596-1598.
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