Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits

Na Young Kim, Hwi Seong Jeon, Myoung Hoon Lee, Ahra Cha, Dong Sook Lee, Hye Jung Lee, Hye Gi Kim, Hyoshin Lee, Young Im Choi, Ohkmae Kim

Research output: Contribution to journalArticle

Abstract

An Arabidopsis small G protein, RabG3b, has been previously characterized as a positive regulator of xylem development in Arabidopsis. Transgenic poplars overexpressing a constitutively active form of RabG3b (RabG3bCA) were developed, and their performance as potential biomass crops was evaluated in the field in Suwon, Korea (37°26′N, 126°98′E) from May to October 2016. RabG3bCA transgenic poplars showed increased stem growth and produced 3–4 times greater dry biomass than hybrid control poplars. Fiber length and diameter were also increased by 15–26% and 28–38%, respectively. Transgenic poplars were analyzed for enzymatic digestibility and biofuel production after chemical pretreatments. Glucose and ethanol yields were higher in pretreated transgenic poplars than hybrid control, and the pretreatment effectiveness of sodium hydroxide was better than that of the other chemicals. We propose that RabG3bCA can add value to the development of biomass crops.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalBiomass and Bioenergy
Volume109
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

G-proteins
Biomass
genetically modified organisms
Proteins
Crops
protein
biomass
energy crops
crop
pretreatment
Arabidopsis
digestibility
Biofuels
xylem
biofuel
hydroxide
Glucose
ethanol
sodium hydroxide
glucose

Keywords

  • Arabidopsis
  • Biofuel
  • Biomass
  • RabG3b
  • Transgenic poplar
  • Xylem development

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

Cite this

Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits. / Kim, Na Young; Jeon, Hwi Seong; Lee, Myoung Hoon; Cha, Ahra; Lee, Dong Sook; Lee, Hye Jung; Kim, Hye Gi; Lee, Hyoshin; Choi, Young Im; Kim, Ohkmae.

In: Biomass and Bioenergy, Vol. 109, 01.02.2018, p. 16-22.

Research output: Contribution to journalArticle

Kim, Na Young ; Jeon, Hwi Seong ; Lee, Myoung Hoon ; Cha, Ahra ; Lee, Dong Sook ; Lee, Hye Jung ; Kim, Hye Gi ; Lee, Hyoshin ; Choi, Young Im ; Kim, Ohkmae. / Field evaluation of transgenic poplars expressing the constitutively active small G protein for improved biomass traits. In: Biomass and Bioenergy. 2018 ; Vol. 109. pp. 16-22.
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