Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis

Yoo Soo Shin, Kyong Hwan Bang, Dong Soo In, Ok Tae Kim, Dong Yoon Hyun, In Ok Ahn, Cho Ku Bon, Suk Weon Kim, Nak Sul Seong, Seon Woo Cha, Dongho Lee, Hyung Kyoon Choi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.

Original languageEnglish
Pages (from-to)1625-1628
Number of pages4
JournalArchives of Pharmacal Research
Volume30
Issue number12
Publication statusPublished - 2007 Dec 31

Fingerprint

Panax
Principal Component Analysis
Principal component analysis
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Metabolomics
Discriminant Analysis
Discriminant analysis
Metabolites
Fresh Water
Sugars
Quality Control
Authentication
Quality control
Multivariate Analysis
Proton Magnetic Resonance Spectroscopy
Water

Keywords

  • H-NMR spectroscopy
  • Fingerprinting analysis
  • Ginseng root
  • Principal component analysis

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Shin, Y. S., Bang, K. H., In, D. S., Kim, O. T., Hyun, D. Y., Ahn, I. O., ... Choi, H. K. (2007). Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis. Archives of Pharmacal Research, 30(12), 1625-1628.

Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis. / Shin, Yoo Soo; Bang, Kyong Hwan; In, Dong Soo; Kim, Ok Tae; Hyun, Dong Yoon; Ahn, In Ok; Bon, Cho Ku; Kim, Suk Weon; Seong, Nak Sul; Cha, Seon Woo; Lee, Dongho; Choi, Hyung Kyoon.

In: Archives of Pharmacal Research, Vol. 30, No. 12, 31.12.2007, p. 1625-1628.

Research output: Contribution to journalArticle

Shin, YS, Bang, KH, In, DS, Kim, OT, Hyun, DY, Ahn, IO, Bon, CK, Kim, SW, Seong, NS, Cha, SW, Lee, D & Choi, HK 2007, 'Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis', Archives of Pharmacal Research, vol. 30, no. 12, pp. 1625-1628.
Shin, Yoo Soo ; Bang, Kyong Hwan ; In, Dong Soo ; Kim, Ok Tae ; Hyun, Dong Yoon ; Ahn, In Ok ; Bon, Cho Ku ; Kim, Suk Weon ; Seong, Nak Sul ; Cha, Seon Woo ; Lee, Dongho ; Choi, Hyung Kyoon. / Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis. In: Archives of Pharmacal Research. 2007 ; Vol. 30, No. 12. pp. 1625-1628.
@article{2160e48c2d754bfe93bee1218a87c18a,
title = "Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis",
abstract = "Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6{\%} of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9{\%} discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.",
keywords = "H-NMR spectroscopy, Fingerprinting analysis, Ginseng root, Principal component analysis",
author = "Shin, {Yoo Soo} and Bang, {Kyong Hwan} and In, {Dong Soo} and Kim, {Ok Tae} and Hyun, {Dong Yoon} and Ahn, {In Ok} and Bon, {Cho Ku} and Kim, {Suk Weon} and Seong, {Nak Sul} and Cha, {Seon Woo} and Dongho Lee and Choi, {Hyung Kyoon}",
year = "2007",
month = "12",
day = "31",
language = "English",
volume = "30",
pages = "1625--1628",
journal = "Archives of Pharmacal Research",
issn = "0253-6269",
publisher = "Pharmaceutical Society of Korea",
number = "12",

}

TY - JOUR

T1 - Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis

AU - Shin, Yoo Soo

AU - Bang, Kyong Hwan

AU - In, Dong Soo

AU - Kim, Ok Tae

AU - Hyun, Dong Yoon

AU - Ahn, In Ok

AU - Bon, Cho Ku

AU - Kim, Suk Weon

AU - Seong, Nak Sul

AU - Cha, Seon Woo

AU - Lee, Dongho

AU - Choi, Hyung Kyoon

PY - 2007/12/31

Y1 - 2007/12/31

N2 - Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.

AB - Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.

KW - H-NMR spectroscopy

KW - Fingerprinting analysis

KW - Ginseng root

KW - Principal component analysis

UR - http://www.scopus.com/inward/record.url?scp=38049121032&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38049121032&partnerID=8YFLogxK

M3 - Article

C2 - 18254251

AN - SCOPUS:38049121032

VL - 30

SP - 1625

EP - 1628

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

SN - 0253-6269

IS - 12

ER -