Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis

Yoo Soo Shin, Kyong Hwan Bang, Dong Soo In, Ok Tae Kim, Dong Yoon Hyun, In Ok Ahn, Cho Ku Bon, Suk Weon Kim, Nak Sul Seong, Seon Woo Cha, Dongho Lee, Hyung Kyoon Choi

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39 Citations (Scopus)

Abstract

Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.

Original languageEnglish
Pages (from-to)1625-1628
Number of pages4
JournalArchives of Pharmacal Research
Volume30
Issue number12
Publication statusPublished - 2007 Dec 31

Keywords

  • H-NMR spectroscopy
  • Fingerprinting analysis
  • Ginseng root
  • Principal component analysis

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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    Shin, Y. S., Bang, K. H., In, D. S., Kim, O. T., Hyun, D. Y., Ahn, I. O., Bon, C. K., Kim, S. W., Seong, N. S., Cha, S. W., Lee, D., & Choi, H. K. (2007). Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis. Archives of Pharmacal Research, 30(12), 1625-1628.