Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis

Yoo Soo Shin, Kyong Hwan Bang, Dong Su In, Jung Sook Sung, Seon Young Kim, Bon Cho Ku, Suk Weon Kim, Dongho Lee, Hyung Kyoon Choi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalBiomolecules and Therapeutics
Volume17
Issue number2
DOIs
Publication statusPublished - 2009 Apr 1

Fingerprint

Astragalus membranaceus
Traditional Medicine
Discriminant Analysis
Discriminant analysis
Principal Component Analysis
Aspartic Acid
Principal component analysis
Nuclear magnetic resonance spectroscopy
Statistical methods
Magnetic Resonance Spectroscopy
Multivariate Analysis
Nuclear magnetic resonance
Expectorants
Aminobutyrates
Immunologic Adjuvants
Metabolomics
Valine
Succinic Acid
Threonine
Metabolites

Keywords

  • H NMR spectroscopy
  • Astragalus membranaceus
  • Canonical discriminant analysis
  • Fingerprinting analysis
  • Principal component analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Drug Discovery
  • Pharmacology

Cite this

Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis. / Shin, Yoo Soo; Bang, Kyong Hwan; In, Dong Su; Sung, Jung Sook; Kim, Seon Young; Ku, Bon Cho; Kim, Suk Weon; Lee, Dongho; Choi, Hyung Kyoon.

In: Biomolecules and Therapeutics, Vol. 17, No. 2, 01.04.2009, p. 133-137.

Research output: Contribution to journalArticle

Shin, Yoo Soo ; Bang, Kyong Hwan ; In, Dong Su ; Sung, Jung Sook ; Kim, Seon Young ; Ku, Bon Cho ; Kim, Suk Weon ; Lee, Dongho ; Choi, Hyung Kyoon. / Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis. In: Biomolecules and Therapeutics. 2009 ; Vol. 17, No. 2. pp. 133-137.
@article{c8408c22252c45ee89712ec6972a38bf,
title = "Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis",
abstract = "The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.",
keywords = "H NMR spectroscopy, Astragalus membranaceus, Canonical discriminant analysis, Fingerprinting analysis, Principal component analysis",
author = "Shin, {Yoo Soo} and Bang, {Kyong Hwan} and In, {Dong Su} and Sung, {Jung Sook} and Kim, {Seon Young} and Ku, {Bon Cho} and Kim, {Suk Weon} and Dongho Lee and Choi, {Hyung Kyoon}",
year = "2009",
month = "4",
day = "1",
doi = "10.4062/biomolther.2009.17.2.133",
language = "English",
volume = "17",
pages = "133--137",
journal = "Biomolecules and Therapeutics",
issn = "1976-9148",
publisher = "Korean Society of Applied Pharmacology",
number = "2",

}

TY - JOUR

T1 - Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis

AU - Shin, Yoo Soo

AU - Bang, Kyong Hwan

AU - In, Dong Su

AU - Sung, Jung Sook

AU - Kim, Seon Young

AU - Ku, Bon Cho

AU - Kim, Suk Weon

AU - Lee, Dongho

AU - Choi, Hyung Kyoon

PY - 2009/4/1

Y1 - 2009/4/1

N2 - The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.

AB - The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.

KW - H NMR spectroscopy

KW - Astragalus membranaceus

KW - Canonical discriminant analysis

KW - Fingerprinting analysis

KW - Principal component analysis

UR - http://www.scopus.com/inward/record.url?scp=69549118369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69549118369&partnerID=8YFLogxK

U2 - 10.4062/biomolther.2009.17.2.133

DO - 10.4062/biomolther.2009.17.2.133

M3 - Article

AN - SCOPUS:69549118369

VL - 17

SP - 133

EP - 137

JO - Biomolecules and Therapeutics

JF - Biomolecules and Therapeutics

SN - 1976-9148

IS - 2

ER -