Abstract
The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.
| Original language | English |
|---|---|
| Pages (from-to) | 133-137 |
| Number of pages | 5 |
| Journal | Biomolecules and Therapeutics |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Apr 1 |
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Keywords
- H NMR spectroscopy
- Astragalus membranaceus
- Canonical discriminant analysis
- Fingerprinting analysis
- Principal component analysis
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Drug Discovery
- Pharmacology
Cite this
Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis. / Shin, Yoo Soo; Bang, Kyong Hwan; In, Dong Su; Sung, Jung Sook; Kim, Seon Young; Ku, Bon Cho; Kim, Suk Weon; Lee, Dongho; Choi, Hyung Kyoon.
In: Biomolecules and Therapeutics, Vol. 17, No. 2, 01.04.2009, p. 133-137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fingerprinting differentiation of Astragalus membranaceus roots according to ages using 1H-NMR spectroscopy and multivariate statistical analysis
AU - Shin, Yoo Soo
AU - Bang, Kyong Hwan
AU - In, Dong Su
AU - Sung, Jung Sook
AU - Kim, Seon Young
AU - Ku, Bon Cho
AU - Kim, Suk Weon
AU - Lee, Dongho
AU - Choi, Hyung Kyoon
PY - 2009/4/1
Y1 - 2009/4/1
N2 - The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.
AB - The root of Astragalus membranaceus is a traditional folk medicine that has been used for many therapeutic purposes in Asia. It reportedly acts as an immunostimulant, tonic, hepatoprotective, diuretic, antidiabetic, analgesic, expectorant, sedative, and anticancer drug. In this study, metabolomic profiling was applied to the roots of A. membranaceus of different ages using NMR coupled with two multivariate statistical analysis methods: such as principal components analysis (PCA) and canonical discriminant analysis (CDA). This allowed various metabolites to be assigned in NMR spectra, including γ-aminobutyric acid (GABA), aspartic acid, succinic acid, glutamic acid, glutamine, N-acetyl aspartic acid, acetic acid, arginine, alanine, threonine, lactic acid, and valine. The score plot from PCA and also CDA allowed a clear separation between samples according to age.
KW - H NMR spectroscopy
KW - Astragalus membranaceus
KW - Canonical discriminant analysis
KW - Fingerprinting analysis
KW - Principal component analysis
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UR - http://www.scopus.com/inward/citedby.url?scp=69549118369&partnerID=8YFLogxK
U2 - 10.4062/biomolther.2009.17.2.133
DO - 10.4062/biomolther.2009.17.2.133
M3 - Article
AN - SCOPUS:69549118369
VL - 17
SP - 133
EP - 137
JO - Biomolecules and Therapeutics
JF - Biomolecules and Therapeutics
SN - 1976-9148
IS - 2
ER -