First report of Penicillium georgiense as a fungal pathogen of onion (Allium cepa L.)

Ji Yeon Oh, Gyung Deok Han, Jin Ju Jeong, Mee Kyung Sang, Se Chul Chun, Ki Deok Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previously, we identified Aspergillus awamori, Fusarium oxysporum, Penicillium brasilianum, and Rhizopus oryzae as the predominant fungal species in onion bulbs in Korea. In that study, we also observed other minor Penicillium spp., among which a specific species was often isolated from yellowish sunken lesions of the bulbs and usually appeared with A.awamori. Hence, in this study, we identified this unknown Penicillium sp. GR-68 by molecular and morphological characteristics and tested its pathogenicity against onion bulbs. As a result, isolate GR-68 was identified as Penicillium georgiense by phylogenetic analyses using neighbor-joining and maximum-likelihood methods with internal transcribed spacer (ITS) and β-tubulin region sequences. When the micro- and macro-morphological characteristics of isolate GR-68 were compared with those of the reference isolate NRRL 35509T of P.georgiense, isolate GR-68 was similar to the reference isolate. In the pathogenicity tests, isolates GR-68 and NRRL 35509T were found to be pathogenic to onion bulbs, where hole-inoculated bulbs showed symptoms of weak yellow halos or yellowish sunken lesions. In addition, the fungal isolates were re-isolated from the symptomatic lesions on the inoculated bulbs and re-confirmed as P.georgiense by the ITS and β-tubulin region sequence analyses. To the best of our knowledge, this is the first report of P.georgiense as a fungal pathogen of onion bulbs.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalCrop Protection
Volume72
DOIs
Publication statusPublished - 2015 Jun 1

Fingerprint

Allium cepa
Penicillium
onions
bulbs
pathogens
tubulin
internal transcribed spacers
pathogenicity
Aspergillus awamori
Rhizopus oryzae
Fusarium oxysporum
Korean Peninsula
phylogeny

Keywords

  • Allium cepa L.
  • Fungal identification
  • Onion
  • Penicillium georgiense
  • Postharvest disease

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

First report of Penicillium georgiense as a fungal pathogen of onion (Allium cepa L.). / Oh, Ji Yeon; Han, Gyung Deok; Jeong, Jin Ju; Sang, Mee Kyung; Chun, Se Chul; Kim, Ki Deok.

In: Crop Protection, Vol. 72, 01.06.2015, p. 83-89.

Research output: Contribution to journalArticle

Oh, Ji Yeon ; Han, Gyung Deok ; Jeong, Jin Ju ; Sang, Mee Kyung ; Chun, Se Chul ; Kim, Ki Deok. / First report of Penicillium georgiense as a fungal pathogen of onion (Allium cepa L.). In: Crop Protection. 2015 ; Vol. 72. pp. 83-89.
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abstract = "Previously, we identified Aspergillus awamori, Fusarium oxysporum, Penicillium brasilianum, and Rhizopus oryzae as the predominant fungal species in onion bulbs in Korea. In that study, we also observed other minor Penicillium spp., among which a specific species was often isolated from yellowish sunken lesions of the bulbs and usually appeared with A.awamori. Hence, in this study, we identified this unknown Penicillium sp. GR-68 by molecular and morphological characteristics and tested its pathogenicity against onion bulbs. As a result, isolate GR-68 was identified as Penicillium georgiense by phylogenetic analyses using neighbor-joining and maximum-likelihood methods with internal transcribed spacer (ITS) and β-tubulin region sequences. When the micro- and macro-morphological characteristics of isolate GR-68 were compared with those of the reference isolate NRRL 35509T of P.georgiense, isolate GR-68 was similar to the reference isolate. In the pathogenicity tests, isolates GR-68 and NRRL 35509T were found to be pathogenic to onion bulbs, where hole-inoculated bulbs showed symptoms of weak yellow halos or yellowish sunken lesions. In addition, the fungal isolates were re-isolated from the symptomatic lesions on the inoculated bulbs and re-confirmed as P.georgiense by the ITS and β-tubulin region sequence analyses. To the best of our knowledge, this is the first report of P.georgiense as a fungal pathogen of onion bulbs.",
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AB - Previously, we identified Aspergillus awamori, Fusarium oxysporum, Penicillium brasilianum, and Rhizopus oryzae as the predominant fungal species in onion bulbs in Korea. In that study, we also observed other minor Penicillium spp., among which a specific species was often isolated from yellowish sunken lesions of the bulbs and usually appeared with A.awamori. Hence, in this study, we identified this unknown Penicillium sp. GR-68 by molecular and morphological characteristics and tested its pathogenicity against onion bulbs. As a result, isolate GR-68 was identified as Penicillium georgiense by phylogenetic analyses using neighbor-joining and maximum-likelihood methods with internal transcribed spacer (ITS) and β-tubulin region sequences. When the micro- and macro-morphological characteristics of isolate GR-68 were compared with those of the reference isolate NRRL 35509T of P.georgiense, isolate GR-68 was similar to the reference isolate. In the pathogenicity tests, isolates GR-68 and NRRL 35509T were found to be pathogenic to onion bulbs, where hole-inoculated bulbs showed symptoms of weak yellow halos or yellowish sunken lesions. In addition, the fungal isolates were re-isolated from the symptomatic lesions on the inoculated bulbs and re-confirmed as P.georgiense by the ITS and β-tubulin region sequence analyses. To the best of our knowledge, this is the first report of P.georgiense as a fungal pathogen of onion bulbs.

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