Fisetin up-regulates the expression of adiponectin in 3t3-l1 adipocytes via the activation of silent mating type information regulation 2 homologue 1 (SIRT1)-deacetylase and peroxisome proliferator-activated receptors (PPARs)

Taewon Jin, Oh Yoen Kim, Min-Jeong Shin, Eun Young Choi, Sung Sook Lee, Ye Sun Han, Ji Hyung Chung

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Adiponectin, an adipokine, has been described as showing physiological benefits against obesity-related malfunctions and vascular dysfunction. Several natural compounds that promote the expression and secretion of adipokines in adipocytes could be useful for treating metabolic disorders. This study investigated the effect of fisetin, a dietary flavonoid, on the regulation of adiponectin in adipocytes using 3T3-L1 preadipocytes. The expression and secretion of adiponectin increased in 3T3-L1 cells upon treatment with fisetin in a dose-dependent manner. Fisetin-induced adiponectin secretion was inhibited by peroxisome proliferator-activated receptor (PPAR) antagonists. It was also revealed that fisetin increased the activities of PPARs and silent mating type information regulation 2 homologue 1 (SIRT1) in a dose-dependent manner. Furthermore, the up-regulation of adiponectin and the activation of PPARs induced by fisetin were prevented by a SIRT1 inhibitor. Fisetin also promoted deacetylation of PPAR γ coactivator 1 (PGC-1) and its interaction with PPARs. SIRT knockdown by siRNA significantly decreased both adiponectin production and PPARs-PGC-1 interaction. These results provide evidence that fisetin promotes the gene expression of adiponectin through the activation of SIRT1 and PPARs in adipocytes.

Original languageEnglish
Pages (from-to)10468-10474
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number43
DOIs
Publication statusPublished - 2014 Oct 29

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Sirtuin 1
Peroxisome Proliferator-Activated Receptors
adiponectin
Adiponectin
adipocytes
Adipocytes
Up-Regulation
Chemical activation
adipokines
Adipokines
secretion
3T3-L1 Cells
metabolic diseases
dosage
small interfering RNA
fisetin
mating types
peroxisome proliferator-activated receptors
Flavonoids
blood vessels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Fisetin up-regulates the expression of adiponectin in 3t3-l1 adipocytes via the activation of silent mating type information regulation 2 homologue 1 (SIRT1)-deacetylase and peroxisome proliferator-activated receptors (PPARs). / Jin, Taewon; Kim, Oh Yoen; Shin, Min-Jeong; Choi, Eun Young; Lee, Sung Sook; Han, Ye Sun; Chung, Ji Hyung.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 43, 29.10.2014, p. 10468-10474.

Research output: Contribution to journalArticle

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