Abstract
A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c and/or C17 : 1ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076T (=KACC 18706T=JCM 31219T).
| Original language | English |
|---|---|
| Article number | 001728 |
| Pages (from-to) | 988-992 |
| Number of pages | 5 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 67 |
| Issue number | 4 |
| DOIs |
|
| Publication status | Published - 2017 Apr 1 |
Fingerprint
Keywords
- Bacteriodetes
- Flavobacterium
- Flavobacterium crassostreae
ASJC Scopus subject areas
- Microbiology
- Ecology, Evolution, Behavior and Systematics
Cite this
Flavobacterium crassostreae sp. nov., isolated from Pacific oyster. / Choi, Sungmi; Shin, Su Kyoung; Kim, Eunji; Yi, Hana.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 4, 001728, 01.04.2017, p. 988-992.Research output: Contribution to journal › Comment/debate
}
TY - JOUR
T1 - Flavobacterium crassostreae sp. nov., isolated from Pacific oyster
AU - Choi, Sungmi
AU - Shin, Su Kyoung
AU - Kim, Eunji
AU - Yi, Hana
PY - 2017/4/1
Y1 - 2017/4/1
N2 - A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c and/or C17 : 1ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076T (=KACC 18706T=JCM 31219T).
AB - A yellow, rod-shaped, Gram-reaction-negative bacterial strain, designated LPB0076T, was isolated from a Pacific oyster. Results of 16S rRNA gene sequence analyses indicated that the strain represented a member of the genus Flavobacterium. It had the highest sequence similarity to the type strains of Flavobacterium frigidarium (97.6 %) and Flavobacterium omnivorum (97.0 %), and its similarities with all other species of the genus Flavobacterium were below 97.0 %. Its genome size (3.02 Mb), DNA G+C content (36.0 mol%), predominant cellular fatty acids (anteiso-C15 : 0, iso-C15 : 0 and C16 : 1ω7c and/or C17 : 1ω6c), and major polar lipid (phosphatidylethanolamine) were similar to those described previously for members of the genus Flavobacterium. In contrast, a number of phenotypic characteristics, including the inability to grow microaerophilically, absence of flexirubin-type pigments and gliding motility and differences in enzymatic reactions, clearly distinguished LPB0076T from other species of the genus Flavobacterium. The polyphasic data presented in this study indicate that this isolate should be classified as representing a novel species of the genus Flavobacterium. The name Flavobacterium crassostreae sp. nov. is therefore proposed for the isolate, with the type strain being LPB0076T (=KACC 18706T=JCM 31219T).
KW - Bacteriodetes
KW - Flavobacterium
KW - Flavobacterium crassostreae
UR - http://www.scopus.com/inward/record.url?scp=85019030642&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019030642&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001728
DO - 10.1099/ijsem.0.001728
M3 - Comment/debate
C2 - 27959776
AN - SCOPUS:85019030642
VL - 67
SP - 988
EP - 992
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 4
M1 - 001728
ER -