Flavobacterium fluvii sp. nov., isolated from stream sediment

An aerobic, yellow-pigmented, Gram-staining-negative bacterium, designated strain H7^T, was isolated from sediment of Gazwa stream in Jinju city, South Korea. Cells of strain H7^T were nonmotile, straight rods that produced flexirubin pigments and showed catalase- and oxidase activities. Strain H7^T contained iso-C_15:0, anteiso-C_15:0 and summed feature 3 (comprising C_16:1ω7c and/or iso-C₁:_5:0 2-OH) as the main fatty acids and menaquinone-6 (MK-6) as the major isoprenoid quinone. The DNA G+C content of strain H7^T was 37.2 mol%. Comparative 16S rRNA gene sequence analysis showed that strain H7^T formed a distinct phyletic line within the genus Flavobacterium. Based on 16S rRNA gene sequence similarities, the novel strain was most closely related to Flavobacterium limicola ST-82^T and Flavobacterium resistens BD-b365^T with 97.2% and 97.0% gene sequence similarities, respectively. The DNA-DNA relatedness of the novel strain with these species was only 18% and 14 %, respectively. On the basis of phenotypic data and molecular properties, strain H7^T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium fluvii sp. nov. is proposed. The type strain is H7^T (=KACC 12818^T=DSM 19978^T).