Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic

Hana Yi, Jongsik Chun

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Two psychrophilic flavobacteria, designated AT1042T and AT1048T, were isolated from terrestrial samples from the Antarctic. Results of 16S rRNA gene sequence analyses indicated a close relationship of these isolates to Flavobacterium flevense (96.9 % similarity for strain AT1042T) and Flavobacterium psychrolimnae (97.0 % for strain AT1048T). Cells were non-motile and non-gliding. Flexirubin-type pigments were absent. Both isolates were psychrophilic, with an optimum and maximum growth temperature of about 15 and 20 °C, respectively. The major isoprenoid quinone, predominant cellular fatty acids and DNA G+C contents (35-37 mol%) were consistent with the placement of the Antarctic isolates in the genus Flavobacterium. Phylogeny based on 16S rRNA gene sequences and several phenotypic characteristics could be used to differentiate these isolates from recognized Flavobacterium species. Despite high 16S rRNA gene sequence similarity (98.9 %) between strains AT1042T and AT1048T, they represented two distinct species as demonstrated by low genomic relatedness (34 %) and a number of differential phenotypic characters. The polyphasic data presented in this study indicated that the new isolates should be classified within two novel species in the genus Flavobacterium. The names Flavobacterium weaverense sp. nov. (type strain AT1042T=IMSNU 14048T=KCTC 12223T=JCM 12384T) and Flavobacterium segetis sp. nov. (type strain AT1048T=IMSNU 14050T=KCTC 12224T=JCM 12385T) are proposed for these Antarctic isolates.

Original languageEnglish
Pages (from-to)1239-1244
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number6
DOIs
Publication statusPublished - 2006 Jun 1
Externally publishedYes

Fingerprint

Flavobacterium
gene
new species
Genes
isoprenoid
rRNA Genes
ribosomal RNA
Flavobacterium flevense
relatedness
nucleotide sequences
pigment
genomics
phylogeny
fatty acid
Flavobacteria
Terpenes
DNA
Growth temperature
Pigments
isoprenoids

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology

Cite this

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abstract = "Two psychrophilic flavobacteria, designated AT1042T and AT1048T, were isolated from terrestrial samples from the Antarctic. Results of 16S rRNA gene sequence analyses indicated a close relationship of these isolates to Flavobacterium flevense (96.9 {\%} similarity for strain AT1042T) and Flavobacterium psychrolimnae (97.0 {\%} for strain AT1048T). Cells were non-motile and non-gliding. Flexirubin-type pigments were absent. Both isolates were psychrophilic, with an optimum and maximum growth temperature of about 15 and 20 °C, respectively. The major isoprenoid quinone, predominant cellular fatty acids and DNA G+C contents (35-37 mol{\%}) were consistent with the placement of the Antarctic isolates in the genus Flavobacterium. Phylogeny based on 16S rRNA gene sequences and several phenotypic characteristics could be used to differentiate these isolates from recognized Flavobacterium species. Despite high 16S rRNA gene sequence similarity (98.9 {\%}) between strains AT1042T and AT1048T, they represented two distinct species as demonstrated by low genomic relatedness (34 {\%}) and a number of differential phenotypic characters. The polyphasic data presented in this study indicated that the new isolates should be classified within two novel species in the genus Flavobacterium. The names Flavobacterium weaverense sp. nov. (type strain AT1042T=IMSNU 14048T=KCTC 12223T=JCM 12384T) and Flavobacterium segetis sp. nov. (type strain AT1048T=IMSNU 14050T=KCTC 12224T=JCM 12385T) are proposed for these Antarctic isolates.",
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AU - Chun, Jongsik

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N2 - Two psychrophilic flavobacteria, designated AT1042T and AT1048T, were isolated from terrestrial samples from the Antarctic. Results of 16S rRNA gene sequence analyses indicated a close relationship of these isolates to Flavobacterium flevense (96.9 % similarity for strain AT1042T) and Flavobacterium psychrolimnae (97.0 % for strain AT1048T). Cells were non-motile and non-gliding. Flexirubin-type pigments were absent. Both isolates were psychrophilic, with an optimum and maximum growth temperature of about 15 and 20 °C, respectively. The major isoprenoid quinone, predominant cellular fatty acids and DNA G+C contents (35-37 mol%) were consistent with the placement of the Antarctic isolates in the genus Flavobacterium. Phylogeny based on 16S rRNA gene sequences and several phenotypic characteristics could be used to differentiate these isolates from recognized Flavobacterium species. Despite high 16S rRNA gene sequence similarity (98.9 %) between strains AT1042T and AT1048T, they represented two distinct species as demonstrated by low genomic relatedness (34 %) and a number of differential phenotypic characters. The polyphasic data presented in this study indicated that the new isolates should be classified within two novel species in the genus Flavobacterium. The names Flavobacterium weaverense sp. nov. (type strain AT1042T=IMSNU 14048T=KCTC 12223T=JCM 12384T) and Flavobacterium segetis sp. nov. (type strain AT1048T=IMSNU 14050T=KCTC 12224T=JCM 12385T) are proposed for these Antarctic isolates.

AB - Two psychrophilic flavobacteria, designated AT1042T and AT1048T, were isolated from terrestrial samples from the Antarctic. Results of 16S rRNA gene sequence analyses indicated a close relationship of these isolates to Flavobacterium flevense (96.9 % similarity for strain AT1042T) and Flavobacterium psychrolimnae (97.0 % for strain AT1048T). Cells were non-motile and non-gliding. Flexirubin-type pigments were absent. Both isolates were psychrophilic, with an optimum and maximum growth temperature of about 15 and 20 °C, respectively. The major isoprenoid quinone, predominant cellular fatty acids and DNA G+C contents (35-37 mol%) were consistent with the placement of the Antarctic isolates in the genus Flavobacterium. Phylogeny based on 16S rRNA gene sequences and several phenotypic characteristics could be used to differentiate these isolates from recognized Flavobacterium species. Despite high 16S rRNA gene sequence similarity (98.9 %) between strains AT1042T and AT1048T, they represented two distinct species as demonstrated by low genomic relatedness (34 %) and a number of differential phenotypic characters. The polyphasic data presented in this study indicated that the new isolates should be classified within two novel species in the genus Flavobacterium. The names Flavobacterium weaverense sp. nov. (type strain AT1042T=IMSNU 14048T=KCTC 12223T=JCM 12384T) and Flavobacterium segetis sp. nov. (type strain AT1048T=IMSNU 14050T=KCTC 12224T=JCM 12385T) are proposed for these Antarctic isolates.

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