Flavonoid compounds are enriched in Lemon balm (Melissa officinalis) leaves by a high level of sucrose and confer increased antioxidant activity

Md Aktar Hossain, Sooah Kim, Kyoung Heon Kim, Sung-Joon Lee, Hojoung Lee

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11 Citations (Scopus)

Abstract

Medicinal plants are widely used in traditional medicine because plant secondary metabolites have been shown to benefit a broad spectrum of health conditions. Lemon balm, Melissa officinalis L., a member of the mint family, is native to Europe and is well known for its ability to reduce stress and anxiety, promote sleep, and ease pain and discomfort associated with digestion. In various plant species, strong anthocyanin induction is triggered by sucrose, but not by other sugars or osmotic stress; however, the mechanisms that induce anthocyanin accumulation in lemon balm leaves in response to sucrose and phytohormones remain unclear. In this study, we investigated the mechanisms that lead to increased levels of flavonoids in lemon balm plants. We observed that sucrose significantly increases the level of flavonoids in lemon balm plants and that sucrose induction appears to be mediated by the phytohormones abscisic acid and ethylene. We also identified delphinidin as the anthocyanidin that is primarily enriched in leaves grown in high-sucrose medium. Finally, we observed that reactive oxygen species levels are positively correlated with sucrose-mediated anthocyanin accumulation. Taken together, our results demonstrate that the level of flavonoids in lemon balm can be increased significantly and that plants such as lemon balm could potentially be used to prevent diseases that have been purported to be caused by free radical damage. Chemical abbreviations used: ABA, (+)-cis, transabscissic acid; ACC, 1-aminocyclopropanecarboxylic acid; CHI, chalcone isomerase; CHS, chalcone synthase; DPPH, 2, 2-diphenyl-1-picrylhydrazyl; GA, gibberellic acid; IAA, indole-3-acetic acid.

Original languageEnglish
Pages (from-to)1907-1913
Number of pages7
JournalHortScience
Volume44
Issue number7
Publication statusPublished - 2009 Dec 1

Fingerprint

lemon balm
Melissa officinalis
flavonoids
antioxidant activity
sucrose
anthocyanins
leaves
1-aminocyclopropane-1-carboxylic acid
plant hormones
indole acetic acid
abscisic acid
chalcone isomerase
delphinidin
anthocyanidins
mint
naringenin-chalcone synthase
anxiety
traditional medicine
osmotic stress
sleep

Keywords

  • Anthocyanins
  • Antioxidant activity
  • Flavonoid
  • Melissa officinalis
  • Phytohormones
  • Principal components
  • Sucrose concentrations

ASJC Scopus subject areas

  • Horticulture

Cite this

@article{3424941acc35416eb30682539c99ad18,
title = "Flavonoid compounds are enriched in Lemon balm (Melissa officinalis) leaves by a high level of sucrose and confer increased antioxidant activity",
abstract = "Medicinal plants are widely used in traditional medicine because plant secondary metabolites have been shown to benefit a broad spectrum of health conditions. Lemon balm, Melissa officinalis L., a member of the mint family, is native to Europe and is well known for its ability to reduce stress and anxiety, promote sleep, and ease pain and discomfort associated with digestion. In various plant species, strong anthocyanin induction is triggered by sucrose, but not by other sugars or osmotic stress; however, the mechanisms that induce anthocyanin accumulation in lemon balm leaves in response to sucrose and phytohormones remain unclear. In this study, we investigated the mechanisms that lead to increased levels of flavonoids in lemon balm plants. We observed that sucrose significantly increases the level of flavonoids in lemon balm plants and that sucrose induction appears to be mediated by the phytohormones abscisic acid and ethylene. We also identified delphinidin as the anthocyanidin that is primarily enriched in leaves grown in high-sucrose medium. Finally, we observed that reactive oxygen species levels are positively correlated with sucrose-mediated anthocyanin accumulation. Taken together, our results demonstrate that the level of flavonoids in lemon balm can be increased significantly and that plants such as lemon balm could potentially be used to prevent diseases that have been purported to be caused by free radical damage. Chemical abbreviations used: ABA, (+)-cis, transabscissic acid; ACC, 1-aminocyclopropanecarboxylic acid; CHI, chalcone isomerase; CHS, chalcone synthase; DPPH, 2, 2-diphenyl-1-picrylhydrazyl; GA, gibberellic acid; IAA, indole-3-acetic acid.",
keywords = "Anthocyanins, Antioxidant activity, Flavonoid, Melissa officinalis, Phytohormones, Principal components, Sucrose concentrations",
author = "Hossain, {Md Aktar} and Sooah Kim and Kim, {Kyoung Heon} and Sung-Joon Lee and Hojoung Lee",
year = "2009",
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language = "English",
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pages = "1907--1913",
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TY - JOUR

T1 - Flavonoid compounds are enriched in Lemon balm (Melissa officinalis) leaves by a high level of sucrose and confer increased antioxidant activity

AU - Hossain, Md Aktar

AU - Kim, Sooah

AU - Kim, Kyoung Heon

AU - Lee, Sung-Joon

AU - Lee, Hojoung

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Medicinal plants are widely used in traditional medicine because plant secondary metabolites have been shown to benefit a broad spectrum of health conditions. Lemon balm, Melissa officinalis L., a member of the mint family, is native to Europe and is well known for its ability to reduce stress and anxiety, promote sleep, and ease pain and discomfort associated with digestion. In various plant species, strong anthocyanin induction is triggered by sucrose, but not by other sugars or osmotic stress; however, the mechanisms that induce anthocyanin accumulation in lemon balm leaves in response to sucrose and phytohormones remain unclear. In this study, we investigated the mechanisms that lead to increased levels of flavonoids in lemon balm plants. We observed that sucrose significantly increases the level of flavonoids in lemon balm plants and that sucrose induction appears to be mediated by the phytohormones abscisic acid and ethylene. We also identified delphinidin as the anthocyanidin that is primarily enriched in leaves grown in high-sucrose medium. Finally, we observed that reactive oxygen species levels are positively correlated with sucrose-mediated anthocyanin accumulation. Taken together, our results demonstrate that the level of flavonoids in lemon balm can be increased significantly and that plants such as lemon balm could potentially be used to prevent diseases that have been purported to be caused by free radical damage. Chemical abbreviations used: ABA, (+)-cis, transabscissic acid; ACC, 1-aminocyclopropanecarboxylic acid; CHI, chalcone isomerase; CHS, chalcone synthase; DPPH, 2, 2-diphenyl-1-picrylhydrazyl; GA, gibberellic acid; IAA, indole-3-acetic acid.

AB - Medicinal plants are widely used in traditional medicine because plant secondary metabolites have been shown to benefit a broad spectrum of health conditions. Lemon balm, Melissa officinalis L., a member of the mint family, is native to Europe and is well known for its ability to reduce stress and anxiety, promote sleep, and ease pain and discomfort associated with digestion. In various plant species, strong anthocyanin induction is triggered by sucrose, but not by other sugars or osmotic stress; however, the mechanisms that induce anthocyanin accumulation in lemon balm leaves in response to sucrose and phytohormones remain unclear. In this study, we investigated the mechanisms that lead to increased levels of flavonoids in lemon balm plants. We observed that sucrose significantly increases the level of flavonoids in lemon balm plants and that sucrose induction appears to be mediated by the phytohormones abscisic acid and ethylene. We also identified delphinidin as the anthocyanidin that is primarily enriched in leaves grown in high-sucrose medium. Finally, we observed that reactive oxygen species levels are positively correlated with sucrose-mediated anthocyanin accumulation. Taken together, our results demonstrate that the level of flavonoids in lemon balm can be increased significantly and that plants such as lemon balm could potentially be used to prevent diseases that have been purported to be caused by free radical damage. Chemical abbreviations used: ABA, (+)-cis, transabscissic acid; ACC, 1-aminocyclopropanecarboxylic acid; CHI, chalcone isomerase; CHS, chalcone synthase; DPPH, 2, 2-diphenyl-1-picrylhydrazyl; GA, gibberellic acid; IAA, indole-3-acetic acid.

KW - Anthocyanins

KW - Antioxidant activity

KW - Flavonoid

KW - Melissa officinalis

KW - Phytohormones

KW - Principal components

KW - Sucrose concentrations

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