Abstract
Many studies have suggested that dietary flavonoids are anticancer agents that induce the apoptosis of cancer cells. However, the effects of flavonoids on the induction of apoptosis in osteosarcoma cells are unclear. Previously, a flavonoid fraction, consisting mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, herein named RCMF (the RVS chloroform-methanol fraction), was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS). This study evaluated the effects of RCMF on the proliferation and apoptosis using human osteosarcoma (HOS) cells. The mechanism of growth inhibition of the HOS cells by the flavonoid fraction, RCMF, was also assessed. The results demonstrated that RCMF exhibited sensitive growth inhibition and induced apoptosis in HOS cells. PARP cleavage was closely associated with the RCMF-induced apoptosis of the HOS cells. Furthermore, the activation of caspase 8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are believed to be involved in the RCMF-mediated apoptosis. Collectively, these findings suggest that RCMF is an agent which may be capable of inducing sensitive growth inhibition and apoptosis in HOS cells.
| Original language | English |
|---|---|
| Pages (from-to) | 309-316 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - General Subjects |
| Volume | 1726 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2005 Nov 30 |
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Keywords
- Apoptosis
- Flavonoid
- Growth inhibition
- Human osteosarcoma cell
- Rhus verniciflua Stokes
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
Cite this
Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells. / Jang, Hyon Seok; Kook, Sung Ho; Son, Young Ok; Kim, Jong Ghee; Jeon, Young Mi; Jang, Yong Suk; Choi, Ki Choon; Kim, Ju; Han, Seong Kyu; Lee, Kyung Yeol; Park, Byung Keon; Cho, Nam Pyo; Lee, Jeong Chae.
In: Biochimica et Biophysica Acta - General Subjects, Vol. 1726, No. 3, 30.11.2005, p. 309-316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flavonoids purified from Rhus verniciflua Stokes actively inhibit cell growth and induce apoptosis in human osteosarcoma cells
AU - Jang, Hyon Seok
AU - Kook, Sung Ho
AU - Son, Young Ok
AU - Kim, Jong Ghee
AU - Jeon, Young Mi
AU - Jang, Yong Suk
AU - Choi, Ki Choon
AU - Kim, Ju
AU - Han, Seong Kyu
AU - Lee, Kyung Yeol
AU - Park, Byung Keon
AU - Cho, Nam Pyo
AU - Lee, Jeong Chae
PY - 2005/11/30
Y1 - 2005/11/30
N2 - Many studies have suggested that dietary flavonoids are anticancer agents that induce the apoptosis of cancer cells. However, the effects of flavonoids on the induction of apoptosis in osteosarcoma cells are unclear. Previously, a flavonoid fraction, consisting mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, herein named RCMF (the RVS chloroform-methanol fraction), was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS). This study evaluated the effects of RCMF on the proliferation and apoptosis using human osteosarcoma (HOS) cells. The mechanism of growth inhibition of the HOS cells by the flavonoid fraction, RCMF, was also assessed. The results demonstrated that RCMF exhibited sensitive growth inhibition and induced apoptosis in HOS cells. PARP cleavage was closely associated with the RCMF-induced apoptosis of the HOS cells. Furthermore, the activation of caspase 8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are believed to be involved in the RCMF-mediated apoptosis. Collectively, these findings suggest that RCMF is an agent which may be capable of inducing sensitive growth inhibition and apoptosis in HOS cells.
AB - Many studies have suggested that dietary flavonoids are anticancer agents that induce the apoptosis of cancer cells. However, the effects of flavonoids on the induction of apoptosis in osteosarcoma cells are unclear. Previously, a flavonoid fraction, consisting mainly of protocatechuic acid, fustin, fisetin, sulfuretin, and butein, herein named RCMF (the RVS chloroform-methanol fraction), was prepared from a crude acetone extract of Rhus verniciflua Stokes (RVS). This study evaluated the effects of RCMF on the proliferation and apoptosis using human osteosarcoma (HOS) cells. The mechanism of growth inhibition of the HOS cells by the flavonoid fraction, RCMF, was also assessed. The results demonstrated that RCMF exhibited sensitive growth inhibition and induced apoptosis in HOS cells. PARP cleavage was closely associated with the RCMF-induced apoptosis of the HOS cells. Furthermore, the activation of caspase 8 and Bax, the inhibition of Bcl-2 expression, and the release of cytochrome c are believed to be involved in the RCMF-mediated apoptosis. Collectively, these findings suggest that RCMF is an agent which may be capable of inducing sensitive growth inhibition and apoptosis in HOS cells.
KW - Apoptosis
KW - Flavonoid
KW - Growth inhibition
KW - Human osteosarcoma cell
KW - Rhus verniciflua Stokes
UR - http://www.scopus.com/inward/record.url?scp=27944448516&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27944448516&partnerID=8YFLogxK
U2 - 10.1016/j.bbagen.2005.08.010
DO - 10.1016/j.bbagen.2005.08.010
M3 - Article
VL - 1726
SP - 309
EP - 316
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0304-4165
IS - 3
ER -