Florigen sequestration in cellular membranes modulates temperature-responsive flowering

Hendry Susila; Snježana Jurić; Lu Liu; Katarzyna Gawarecka; Kyung Sook Chung; Suhyun Jin; Soo Jin Kim; Zeeshan Nasim; Geummin Youn; Mi Chung Suh; Hao Yu; Ji Hoon Ahn

doi:10.1126/science.abh4054

Florigen sequestration in cellular membranes modulates temperature-responsive flowering

Hendry Susila, Snježana Jurić, Lu Liu, Katarzyna Gawarecka, Kyung Sook Chung, Suhyun Jin, Soo Jin Kim, Zeeshan Nasim, Geummin Youn, Mi Chung Suh, Hao Yu, Ji Hoon Ahn

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Plants respond to temperature changes by modulating florigen activity to optimize the timing of flowering. We show that the Arabidopsis thaliana mobile florigen FLOWERING LOCUS T (FT) interacts with the negatively charged phospholipid phosphatidylglycerol (PG) at cellular membranes and binds the lipid bilayer. Perturbing PG biosynthesis in phloem companion cells leads to temperature-insensitive early flowering. Low temperatures facilitate FT sequestration in the cellular membrane of the companion cell, thus reducing soluble FT levels and delaying flowering. A mutant in PHOSPHATIDYLGLYCEROLPHOSPHATE SYNTHASE 1 accumulates more soluble FT at lower temperatures and exhibits reduced temperature sensitivity. Thus, cellular membranes sequester FT through their ability to bind the phospholipid PG, and this sequestration modulates the plant’s response to temperature changes.

Original language	English
Pages (from-to)	1137-1142
Number of pages	6
Journal	Science
Volume	373
Issue number	6559
DOIs	https://doi.org/10.1126/science.abh4054
Publication status	Published - 2021 Sept 3

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@article{749c199bef3444dfaed5f16852203f28,

title = "Florigen sequestration in cellular membranes modulates temperature-responsive flowering",

abstract = "Plants respond to temperature changes by modulating florigen activity to optimize the timing of flowering. We show that the Arabidopsis thaliana mobile florigen FLOWERING LOCUS T (FT) interacts with the negatively charged phospholipid phosphatidylglycerol (PG) at cellular membranes and binds the lipid bilayer. Perturbing PG biosynthesis in phloem companion cells leads to temperature-insensitive early flowering. Low temperatures facilitate FT sequestration in the cellular membrane of the companion cell, thus reducing soluble FT levels and delaying flowering. A mutant in PHOSPHATIDYLGLYCEROLPHOSPHATE SYNTHASE 1 accumulates more soluble FT at lower temperatures and exhibits reduced temperature sensitivity. Thus, cellular membranes sequester FT through their ability to bind the phospholipid PG, and this sequestration modulates the plant{\textquoteright}s response to temperature changes.",

author = "Hendry Susila and Snje{\v z}ana Juri{\'c} and Lu Liu and Katarzyna Gawarecka and Chung, {Kyung Sook} and Suhyun Jin and Kim, {Soo Jin} and Zeeshan Nasim and Geummin Youn and Suh, {Mi Chung} and Hao Yu and Ahn, {Ji Hoon}",

note = "Funding Information: This work was supported by a National Research Foundation (NRF) of Korea grant funded by the Korean government (NRF-2017R1A2B3009624 to J.H.A.) and Samsung Science and Technology Foundation (SSTF-BA1602-12 to J.H.A). This work was also supported by the Singapore National Research Foundation Investigatorship Programme (NRFNRFI2016-02 to H.Y). Publisher Copyright: {\textcopyright} 2021 American Association for the Advancement of Science. All rights reserved.",

year = "2021",

month = sep,

day = "3",

doi = "10.1126/science.abh4054",

language = "English",

volume = "373",

pages = "1137--1142",

journal = "Science",

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number = "6559",

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TY - JOUR

T1 - Florigen sequestration in cellular membranes modulates temperature-responsive flowering

AU - Susila, Hendry

AU - Jurić, Snježana

AU - Liu, Lu

AU - Gawarecka, Katarzyna

AU - Chung, Kyung Sook

AU - Jin, Suhyun

AU - Kim, Soo Jin

AU - Nasim, Zeeshan

AU - Youn, Geummin

AU - Suh, Mi Chung

AU - Yu, Hao

AU - Ahn, Ji Hoon

N1 - Funding Information: This work was supported by a National Research Foundation (NRF) of Korea grant funded by the Korean government (NRF-2017R1A2B3009624 to J.H.A.) and Samsung Science and Technology Foundation (SSTF-BA1602-12 to J.H.A). This work was also supported by the Singapore National Research Foundation Investigatorship Programme (NRFNRFI2016-02 to H.Y). Publisher Copyright: © 2021 American Association for the Advancement of Science. All rights reserved.

PY - 2021/9/3

Y1 - 2021/9/3

N2 - Plants respond to temperature changes by modulating florigen activity to optimize the timing of flowering. We show that the Arabidopsis thaliana mobile florigen FLOWERING LOCUS T (FT) interacts with the negatively charged phospholipid phosphatidylglycerol (PG) at cellular membranes and binds the lipid bilayer. Perturbing PG biosynthesis in phloem companion cells leads to temperature-insensitive early flowering. Low temperatures facilitate FT sequestration in the cellular membrane of the companion cell, thus reducing soluble FT levels and delaying flowering. A mutant in PHOSPHATIDYLGLYCEROLPHOSPHATE SYNTHASE 1 accumulates more soluble FT at lower temperatures and exhibits reduced temperature sensitivity. Thus, cellular membranes sequester FT through their ability to bind the phospholipid PG, and this sequestration modulates the plant’s response to temperature changes.

AB - Plants respond to temperature changes by modulating florigen activity to optimize the timing of flowering. We show that the Arabidopsis thaliana mobile florigen FLOWERING LOCUS T (FT) interacts with the negatively charged phospholipid phosphatidylglycerol (PG) at cellular membranes and binds the lipid bilayer. Perturbing PG biosynthesis in phloem companion cells leads to temperature-insensitive early flowering. Low temperatures facilitate FT sequestration in the cellular membrane of the companion cell, thus reducing soluble FT levels and delaying flowering. A mutant in PHOSPHATIDYLGLYCEROLPHOSPHATE SYNTHASE 1 accumulates more soluble FT at lower temperatures and exhibits reduced temperature sensitivity. Thus, cellular membranes sequester FT through their ability to bind the phospholipid PG, and this sequestration modulates the plant’s response to temperature changes.

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U2 - 10.1126/science.abh4054

DO - 10.1126/science.abh4054

M3 - Article

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AN - SCOPUS:85114164196

SN - 0036-8075

VL - 373

SP - 1137

EP - 1142

JO - Science

JF - Science

IS - 6559

ER -

Florigen sequestration in cellular membranes modulates temperature-responsive flowering

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