Flow cytometric enumeration of parasitemia in cultures of plasmodium falciparum stained with SYBR green i and CD235A

Jin Woo Jang, Ju Yeon Kim, Jung Yoon, Soo-Young Yoon, Chy Hyun Cho, Eun Taek Han, Seong Soo A An, Chae Seung Lim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2-37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2-66.0%). High correlation of measured parasitaemia (r = 0.98, P < 0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.

Original languageEnglish
Article number536723
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014 Dec 8

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Parasitemia
Plasmodium falciparum
Microscopic examination
Blood
blood
Cells
detection method
Erythrocytes
SYBR Green I
analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Flow cytometric enumeration of parasitemia in cultures of plasmodium falciparum stained with SYBR green i and CD235A. / Jang, Jin Woo; Kim, Ju Yeon; Yoon, Jung; Yoon, Soo-Young; Cho, Chy Hyun; Han, Eun Taek; An, Seong Soo A; Lim, Chae Seung.

In: Scientific World Journal, Vol. 2014, 536723, 08.12.2014.

Research output: Contribution to journalArticle

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AU - Yoon, Soo-Young

AU - Cho, Chy Hyun

AU - Han, Eun Taek

AU - An, Seong Soo A

AU - Lim, Chae Seung

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