Flow cytometric enumeration of parasitemia in cultures of plasmodium falciparum stained with SYBR green i and CD235A

Jin Woo Jang, Ju Yeon Kim, Jung Yoon, Soo-Young Yoon, Chy Hyun Cho, Eun Taek Han, Seong Soo A An, Chae Seung Lim

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7 Citations (Scopus)

Abstract

A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2-37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2-66.0%). High correlation of measured parasitaemia (r = 0.98, P < 0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.

Original languageEnglish
Article number536723
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014 Dec 8

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

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