Fluorescence-based immunosensor using three-dimensional CNT network structure for sensitive and reproducible detection of oral squamous cell carcinoma biomarker

Chung Kil Song, Eunkyul Oh, Min Sung Kang, Bum Seok Shin, Soo Young Han, Mingi Jung, Eui Seok Lee, Soo-Young Yoon, Myung Mo Sung, Wei Beng Ng, Nam Joon Cho, Haiwon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A hierarchical three-dimensional network of carbon nanotubes on Si pillar substrate (3DN-CNTs) was developed for the accurate detection of oral squamous cell carcinoma (OSCC) in clinical saliva samples. The 3DN-CNTs were uniformly coated with a layer of aluminum oxides to enhance structural stability during biomarker detection. Cytokeratin-19 antigen (Cyfra 21-1) was utilized as a model biomarker of OSCC for fluorescence-based immunosensor using 3DN-CNTs (3DN-CNTs sensor). The 3DN-CNTs sensor enhances the sensitivity of Cyfra 21-1 detection by increasing the density of immobilized antibody through high surface area of 3DN-CNTs and enhancing the accessibility of biomolecules through the ordered pathway of hierarchical structure. The reliable detection limit for sensing of Cyfra 21-1 was estimated as in the level of 0.5 ng/mL and the quantitative estimation of Cyfra 21-1 was analyzed by 4-parameter logistic (4-PL) model for curve-fitting analysis. Clinical applicability of 3DN-CNTs sensor was evaluated through correlation with the commercially available electrochemiluminescence (ECL) detection system in the hospital. The assay results of the two systems for clinical saliva samples showed a good linear correlation. The 3DN-CNTs sensor offers great potential for accurate diagnosis of OSCC using Cyfra 21-1 biomarker in clinical fluids.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalAnalytica Chimica Acta
Volume1027
DOIs
Publication statusPublished - 2018 Oct 16

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Keywords

  • 3DN-CNTs
  • Biosensor
  • Cyfra 21-1
  • Fluorescence-based immunoassay
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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