Abstract
A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin®, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.
| Original language | English |
|---|---|
| Pages (from-to) | 642-647 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 68 |
| DOIs | |
| Publication status | Published - 2015 Jun 5 |
| Externally published | Yes |
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Keywords
- DNA-template silver nanoclusters
- Fluorescence switch
- Fluorescent probes
- Silver ion detection
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters. / Lee, Jihyun; Park, Juhee; Hee Lee, Hong; Park, Hansoo; Kim, Hugh I.; Kim, Won Jong.
In: Biosensors and Bioelectronics, Vol. 68, 05.06.2015, p. 642-647.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters
AU - Lee, Jihyun
AU - Park, Juhee
AU - Hee Lee, Hong
AU - Park, Hansoo
AU - Kim, Hugh I.
AU - Kim, Won Jong
PY - 2015/6/5
Y1 - 2015/6/5
N2 - A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin®, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.
AB - A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin®, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.
KW - DNA-template silver nanoclusters
KW - Fluorescence switch
KW - Fluorescent probes
KW - Silver ion detection
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U2 - 10.1016/j.bios.2015.01.058
DO - 10.1016/j.bios.2015.01.058
M3 - Article
VL - 68
SP - 642
EP - 647
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -