Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

Jihyun Lee, Juhee Park, Hong Hee Lee, Hansoo Park, Hugh I. Kim, Won Jong Kim

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt12-AgNCs by forming a bridge between two Cyt12-AgNCs, where Cyt12 is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt12-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin®, which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions.

Original languageEnglish
Pages (from-to)642-647
Number of pages6
JournalBiosensors and Bioelectronics
Volume68
DOIs
Publication statusPublished - 2015 Jun 5
Externally publishedYes

Fingerprint

Dimerization
Nanoclusters
Silver
DNA
Fluorescence
Switches
Ions
Silver Sulfadiazine
Circular dichroism spectroscopy
Cytosine
Fluorescence Spectrometry
Fluorescence spectroscopy
Ointments
Circular Dichroism
Dimers
Reaction Time
Mass spectrometry
Metal ions
Assays
Mass Spectrometry

Keywords

  • DNA-template silver nanoclusters
  • Fluorescence switch
  • Fluorescent probes
  • Silver ion detection

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters. / Lee, Jihyun; Park, Juhee; Hee Lee, Hong; Park, Hansoo; Kim, Hugh I.; Kim, Won Jong.

In: Biosensors and Bioelectronics, Vol. 68, 05.06.2015, p. 642-647.

Research output: Contribution to journalArticle

Lee, Jihyun ; Park, Juhee ; Hee Lee, Hong ; Park, Hansoo ; Kim, Hugh I. ; Kim, Won Jong. / Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters. In: Biosensors and Bioelectronics. 2015 ; Vol. 68. pp. 642-647.
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