Fluorescence Turn-on Synthetic Lipid Rafts on Supramolecular Sheets and Hierarchical Concanavalin A Assembly

Yongju Kim, Xin Liu, Huichang Li, Myongsoo Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Here we report fluorescence turn-on synthetic lipid rafts by self-assembly of a cationic distyrylanthracene derivative on a negatively-charged sheet in an aqueous solution. First, the negatively-charged 2D membrane structure is formed by lateral associations of aromatic rods with carboxylate groups. Then, the synthetic rafts are floated on the surface of the negatively-charged sheets through electrostatic interactions. The fluorescence of the synthetic rafts is turned on due to the aggregation of the positively-charged AIE dye on the sheets, facilitating monitoring of the formation of rafts. Concanavalin A (Con A) protein can load hierarchically onto the synthetic rafts at neutral pH to provide discrete Con A aggregates with a uniform size of ≈12 nm. The uniform aggregates of Con A on the synthetic rafts can stimulate Jurkat cells with enhanced efficiency, as compared with random-sized aggregates of Con A.

Original languageEnglish
Pages (from-to)952-957
Number of pages6
JournalChemistry - An Asian Journal
Volume14
Issue number7
DOIs
Publication statusPublished - 2019 Apr 1
Externally publishedYes

Keywords

  • aggregation-induced emission
  • protein assembly
  • self-assembly
  • supramolecular sheets
  • synthetic lipid rafts

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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