Fluorogenic pH-sensitive polydiacetylene (PDA) liposomes as a drug carrier

Sang Ho Won, Jong Uk Lee, Sang Jun Sim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A crucial issue for current liposomal carriers in clinical applications is the sustained-release property of the encapsulated drugs. We have developed novel fluorogenic pH-sensitive polymerized liposomes composed of polydiacetylene (PDA) lipids and other types of lipids. Unilamellar liposomes containing 10,12-pentacosadiynoic acid (PCDA), 1,2-dioleoyl-sn-glycero-3- phosphoethanolamine (DOPE), and N-palmitoyl homocysteine (PHC) were loaded with ampicillin. These liposomes fused to each other rapidly when the medium pH was lowered from 7 to 4. The polymerized liposomes were characterized in terms of particle size distribution. The liposome size increased approximately 20-fold from 110.0±19.3 nm to 2046.7±487.4 nm as the pH was lowered. Cross-linking of the diacetylene lipids prevents drug leakage and the encapsulated drug can be instantaneously released at acidic pH condition. The ampicillin was nearly completely released (74.4±3.9%) from liposomes within 4 h under acidic pH conditions and the released amounts of ampicillin were analyzed by HPLC. Finally, the therapeutic effect was observed by the appearance of plaques on a lawn of E. coli, and fluorescent images of the PDA liposomes were taken from the plaques for drug release monitoring. As a result, this research demonstrates that such novel pH-sensitive polymerized liposomes have great prospects as a drug carrier.

Original languageEnglish
Pages (from-to)3792-3800
Number of pages9
JournalJournal of Nanoscience and Nanotechnology
Volume13
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Fingerprint

Drug Carriers
Liposomes
drugs
Ampicillin
lipids
Lipids
Pharmaceutical Preparations
Unilamellar Liposomes
particle size distribution
Drug Monitoring
Therapeutic Uses
polydiacetylene
Particle Size
Particle size analysis
leakage
Escherichia coli
High Pressure Liquid Chromatography
acids
Monitoring
Acids

Keywords

  • Ampicillin
  • Drug Carrier
  • Fluorescence
  • Liposome
  • pH-Sensitive
  • Polydiacetylene

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Fluorogenic pH-sensitive polydiacetylene (PDA) liposomes as a drug carrier. / Won, Sang Ho; Lee, Jong Uk; Sim, Sang Jun.

In: Journal of Nanoscience and Nanotechnology, Vol. 13, No. 6, 01.06.2013, p. 3792-3800.

Research output: Contribution to journalArticle

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