Fluroxypyr-1-methylheptyl ester induced ROS production and mitochondrial apoptosis through the MAPK signaling cascade in porcine trophectoderm and uterine luminal epithelial cells

Miji Kim, Garam An, Whasun Lim, Gwonhwa Song

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

FPMH (Fluroxypyr-1-methylheptyl ester) is a synthetic auxin herbicide used in agriculture. The mechanism by which FPMH induces adverse effects in porcine trophectoderm (pTr) and porcine uterine luminal epithelial (pLE) cells, which are involved in porcine implantation, have not been studied yet. Therefore, the present study investigates the toxicological effects of FPMH on pTr and pLE cells. We confirmed that FPMH induced cytotoxic effects on the cells, including apoptosis induction, mitochondrial membrane potential (MMP) depolarization, and ROS production. The phosphorylation of the MAPK pathway (ERK1/2, JNK, and p38) was dysregulated by FPMH administration. In addition, FPMH could suppress cell-cell adhesion and migration abilities of pTr and pLE, which are crucial for implantation. Therefore, exposure to FPMH induced adverse effects in pTr and pLE cells and could result in implantation failure.

Original languageEnglish
Article number105196
JournalPesticide Biochemistry and Physiology
Volume187
DOIs
Publication statusPublished - 2022 Oct

Keywords

  • Apoptosis
  • Fluroxypyr
  • Mitochondrial membrane potential
  • ROS production
  • Trophectoderm
  • Uterine luminal epithelial

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'Fluroxypyr-1-methylheptyl ester induced ROS production and mitochondrial apoptosis through the MAPK signaling cascade in porcine trophectoderm and uterine luminal epithelial cells'. Together they form a unique fingerprint.

Cite this