Fnr, NarL and NarP regulation and time course expression of Escherichia coll aeg-46.5 gene

Juhyuk Ahn, MuHyeon Choe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The anaerobically expressed gene aeg-46.5, which had been identified by the operon fusion technique with a hybrid bacteriophage of λ and Mu, λplacMu53, was studied for its expression pattern and growth. The expression of aeg-46.5 was studied in the wild-type cell and mutant cells that have mutation (s) in the control gene of anaerobic respiration (fnr) and nitrate response (narL and narP). The β-galactosidase reporter gene showed maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Both 40 mM and 100 mM concentrations of nitrate ion in the medium had little effect on expression level. We propose that aeg-46.5 is subject to multiple regulations of anaerobic activation by Fnr, nitrate activation by NarP and repression mediated by NarL.

Original languageEnglish
Pages (from-to)88-91
Number of pages4
JournalJournal of Biochemistry and Molecular Biology
Volume29
Issue number1
Publication statusPublished - 1996 Jan 31

Fingerprint

Escherichia
Nitrates
Genes
Bacteriophage mu
Chemical activation
Galactosidases
Bacteriophages
Operon
Reporter Genes
Respiration
Fusion reactions
Switches
Ions
Mutation
Growth

Keywords

  • aeg-46.5
  • Anaerobically expressed gene
  • NarL
  • NarP
  • Nitrate induction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Fnr, NarL and NarP regulation and time course expression of Escherichia coll aeg-46.5 gene. / Ahn, Juhyuk; Choe, MuHyeon.

In: Journal of Biochemistry and Molecular Biology, Vol. 29, No. 1, 31.01.1996, p. 88-91.

Research output: Contribution to journalArticle

@article{e786dce1ee61440daee71e188e3c0aac,
title = "Fnr, NarL and NarP regulation and time course expression of Escherichia coll aeg-46.5 gene",
abstract = "The anaerobically expressed gene aeg-46.5, which had been identified by the operon fusion technique with a hybrid bacteriophage of λ and Mu, λplacMu53, was studied for its expression pattern and growth. The expression of aeg-46.5 was studied in the wild-type cell and mutant cells that have mutation (s) in the control gene of anaerobic respiration (fnr) and nitrate response (narL and narP). The β-galactosidase reporter gene showed maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Both 40 mM and 100 mM concentrations of nitrate ion in the medium had little effect on expression level. We propose that aeg-46.5 is subject to multiple regulations of anaerobic activation by Fnr, nitrate activation by NarP and repression mediated by NarL.",
keywords = "aeg-46.5, Anaerobically expressed gene, NarL, NarP, Nitrate induction",
author = "Juhyuk Ahn and MuHyeon Choe",
year = "1996",
month = "1",
day = "31",
language = "English",
volume = "29",
pages = "88--91",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "1",

}

TY - JOUR

T1 - Fnr, NarL and NarP regulation and time course expression of Escherichia coll aeg-46.5 gene

AU - Ahn, Juhyuk

AU - Choe, MuHyeon

PY - 1996/1/31

Y1 - 1996/1/31

N2 - The anaerobically expressed gene aeg-46.5, which had been identified by the operon fusion technique with a hybrid bacteriophage of λ and Mu, λplacMu53, was studied for its expression pattern and growth. The expression of aeg-46.5 was studied in the wild-type cell and mutant cells that have mutation (s) in the control gene of anaerobic respiration (fnr) and nitrate response (narL and narP). The β-galactosidase reporter gene showed maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Both 40 mM and 100 mM concentrations of nitrate ion in the medium had little effect on expression level. We propose that aeg-46.5 is subject to multiple regulations of anaerobic activation by Fnr, nitrate activation by NarP and repression mediated by NarL.

AB - The anaerobically expressed gene aeg-46.5, which had been identified by the operon fusion technique with a hybrid bacteriophage of λ and Mu, λplacMu53, was studied for its expression pattern and growth. The expression of aeg-46.5 was studied in the wild-type cell and mutant cells that have mutation (s) in the control gene of anaerobic respiration (fnr) and nitrate response (narL and narP). The β-galactosidase reporter gene showed maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Both 40 mM and 100 mM concentrations of nitrate ion in the medium had little effect on expression level. We propose that aeg-46.5 is subject to multiple regulations of anaerobic activation by Fnr, nitrate activation by NarP and repression mediated by NarL.

KW - aeg-46.5

KW - Anaerobically expressed gene

KW - NarL

KW - NarP

KW - Nitrate induction

UR - http://www.scopus.com/inward/record.url?scp=0030558885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030558885&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030558885

VL - 29

SP - 88

EP - 91

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 1

ER -