Fnr, NarL and NarP regulation and time course expression of Escherichia coll aeg-46.5 gene

Juhyuk Ahn, Muhyeon Choe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The anaerobically expressed gene aeg-46.5, which had been identified by the operon fusion technique with a hybrid bacteriophage of λ and Mu, λplacMu53, was studied for its expression pattern and growth. The expression of aeg-46.5 was studied in the wild-type cell and mutant cells that have mutation (s) in the control gene of anaerobic respiration (fnr) and nitrate response (narL and narP). The β-galactosidase reporter gene showed maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Both 40 mM and 100 mM concentrations of nitrate ion in the medium had little effect on expression level. We propose that aeg-46.5 is subject to multiple regulations of anaerobic activation by Fnr, nitrate activation by NarP and repression mediated by NarL.

Original languageEnglish
Pages (from-to)88-91
Number of pages4
JournalJournal of Biochemistry and Molecular Biology
Volume29
Issue number1
Publication statusPublished - 1996 Jan 31

Keywords

  • Anaerobically expressed gene
  • NarL
  • NarP
  • Nitrate induction
  • aeg-46.5

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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