Forced expression of HoxB4 enhances hematopoietic differentiation by human embryonic stem cells

Gab Sang Lee, Byung Soo Kim, Jae Hung Sheih, Malcolm A S Moore

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

HoxB4 has been shown to enhance hematopoietic engraftment by hematopoietic stem cells (HSC) from differentiating mouse embryonic stem cell (mESC) cultures. Here we examined the effect of ectopic expression of HoxB4 in differentiated human embryonic stem cells (hESCs). Stable HoxB4-expressing hESCs were established by lentiviral transduction, and the forced expression of HoxB4 did not affect stem cell features. HoxB4-expressing hESC-derived CD34+ cells generated higher numbers of erythroid and blast-like colonies than controls. The number of CD34+ cells increased but CD45+ and KDR+ cell numbers were not significantly affected. When the hESC derived CD34+ cells were transplanted into NOD/ SCIDβ2m-/- mice, the ectopic expression of HoxB4 did not alter their repopulating capacity. Our findings show that overexpression of HoxB4 in differentiating hESCs increases hematopoietic colony formation and hematopoietic cell formation in vitro, but does not affect in vivo repopulation in adult mice hosts.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalMolecules and Cells
Volume25
Issue number4
Publication statusPublished - 2008 Jun 30

Keywords

  • Hematopoietic
  • HoxB4
  • Human embryonic Stem Cells
  • Lentivirus

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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