Forkhead Box M1 is regulated by heat shock factor 1 and promotes glioma cells survival under heat shock stress

Bingbing Dai, Aihua Gong, Zhitao Jing, Kenneth D. Aldape, Shin-Hyuk Kang, Raymond Sawaya, Suyun Huang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Background: FoxM1 plays many roles in cancer development, progression, and cancer survival. Results: FoxM1 is regulated by HSF1 and promotes cell cycle progression and cancer cell survival under heat stress conditions. Conclusion: FoxM1 is critical for cell survival under heat stress condition. Significance: HSF1-FoxM1 is a novel connection between heat shock proteins and stress responses and a novel pathway for cell survival under stress condition. The forkhead box M1 (FoxM1) is a key transcription factor regulating multiple aspects of cell biology. Prior studies have shown that FoxM1 is overexpressed in a variety of human tumors, including brain tumor, and plays a critical role in cancer development and progression. In this study we found that FoxM1 was up-regulated by heat shock factor 1 (HSF1) under heat shock stress condition in multiple cell lines. Knockdown of HSF1 with HSF1 siRNA or inhibition of HSF1 with a HSF1 inhibitor abrogated heat shock-induced expression of FoxM1. Genetic deletion of HSF1 in mouse embryo fibroblast cells also abolished heat shock stress-induced FoxM1 expression. Moreover, we showed that HSF1 directly bound to FoxM1 promoter and increased FoxM1 promoter activity. Furthermore, we demonstrated that FoxM1 was required for theG2-M phase progression through regulating Cdc2, Cdc20, and Cdc25B under a mild heat shock stress but enhanced cell survival under lethal heat shock stress condition. Finally, in human glioblastoma specimens, FoxM1 overexpression correlated with elevated HSF1 expression. Our results indicate that FoxM1 is regulated by HSF1 and is critical for HSF1-mediated heat shock response. We demonstrated a novel mechanism of stress resistance controlled by HSF1 and a new HSF-FoxM1 connection that mediates cellular thermotolerance.

Original languageEnglish
Pages (from-to)1634-1642
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number3
DOIs
Publication statusPublished - 2013 Jan 18
Externally publishedYes

Fingerprint

Glioma
Shock
Cell Survival
Hot Temperature
Cells
Heat-Shock Response
Neoplasms
Tumors
Cytology
Glioblastoma
Heat-Shock Proteins
Brain Neoplasms
Fibroblasts
Cell Division
Small Interfering RNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Forkhead Box M1 is regulated by heat shock factor 1 and promotes glioma cells survival under heat shock stress. / Dai, Bingbing; Gong, Aihua; Jing, Zhitao; Aldape, Kenneth D.; Kang, Shin-Hyuk; Sawaya, Raymond; Huang, Suyun.

In: Journal of Biological Chemistry, Vol. 288, No. 3, 18.01.2013, p. 1634-1642.

Research output: Contribution to journalArticle

Dai, Bingbing ; Gong, Aihua ; Jing, Zhitao ; Aldape, Kenneth D. ; Kang, Shin-Hyuk ; Sawaya, Raymond ; Huang, Suyun. / Forkhead Box M1 is regulated by heat shock factor 1 and promotes glioma cells survival under heat shock stress. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 3. pp. 1634-1642.
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AU - Dai, Bingbing

AU - Gong, Aihua

AU - Jing, Zhitao

AU - Aldape, Kenneth D.

AU - Kang, Shin-Hyuk

AU - Sawaya, Raymond

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AB - Background: FoxM1 plays many roles in cancer development, progression, and cancer survival. Results: FoxM1 is regulated by HSF1 and promotes cell cycle progression and cancer cell survival under heat stress conditions. Conclusion: FoxM1 is critical for cell survival under heat stress condition. Significance: HSF1-FoxM1 is a novel connection between heat shock proteins and stress responses and a novel pathway for cell survival under stress condition. The forkhead box M1 (FoxM1) is a key transcription factor regulating multiple aspects of cell biology. Prior studies have shown that FoxM1 is overexpressed in a variety of human tumors, including brain tumor, and plays a critical role in cancer development and progression. In this study we found that FoxM1 was up-regulated by heat shock factor 1 (HSF1) under heat shock stress condition in multiple cell lines. Knockdown of HSF1 with HSF1 siRNA or inhibition of HSF1 with a HSF1 inhibitor abrogated heat shock-induced expression of FoxM1. Genetic deletion of HSF1 in mouse embryo fibroblast cells also abolished heat shock stress-induced FoxM1 expression. Moreover, we showed that HSF1 directly bound to FoxM1 promoter and increased FoxM1 promoter activity. Furthermore, we demonstrated that FoxM1 was required for theG2-M phase progression through regulating Cdc2, Cdc20, and Cdc25B under a mild heat shock stress but enhanced cell survival under lethal heat shock stress condition. Finally, in human glioblastoma specimens, FoxM1 overexpression correlated with elevated HSF1 expression. Our results indicate that FoxM1 is regulated by HSF1 and is critical for HSF1-mediated heat shock response. We demonstrated a novel mechanism of stress resistance controlled by HSF1 and a new HSF-FoxM1 connection that mediates cellular thermotolerance.

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