Fractionated aged black garlic extracts enhance growth of Anti-My-10 hybridoma cells and production of IgG1 antibody

Ji Young Lee, Namhyun Chung, Yong Kwon Lee

Research output: Contribution to journalArticle

Abstract

Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth-promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.

Original languageEnglish
Pages (from-to)61-63
Number of pages3
JournalJournal of Applied Biological Chemistry
Volume57
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • Aged black garlic
  • Fractionation
  • Hybridoma cell line
  • Mouse igg1 production
  • Suspension culture

ASJC Scopus subject areas

  • Organic Chemistry
  • Bioengineering

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