Fractionation and angiotensin I-converting enzyme (ACE) inhibitory activity of gelatin hydrolysates from by-products of Alaska pollock surimi

Chan Ho Park, Hyung Jun Kim, Kyung Tae Kang, Jae W. Park, Jin Soo Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Gelatin hydrolysates with a high inhibitory activity against angiotensin I-converting enzyme (ACE) were fractionated from Alaska pollock surimi refiner discharge. The ACE-inhibitory activity, expressed as IC50 (mg/mL), was highest (0.49 mg/mL) in gelatin hydrolysates formed by sequential 2-hr treatments of Pronase and Flavourzyme. After fractionation through four different membrane filters with molecular weight cut-offs of 3, 5, 10, and 30 kDa, the highest ACE-inhibitory activity (0.21 mg/mL) was observed with the 3-kDa filtrate.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalFisheries and Aquatic Science
Volume12
Issue number2
Publication statusPublished - 2009 Jun 1
Externally publishedYes

Fingerprint

Theragra chalcogramma
surimi
peptidyl-dipeptidase A
Enzyme activity
Peptidyl-Dipeptidase A
Gelatin
Fractionation
gelatin
hydrolysates
byproducts
enzyme activity
Byproducts
fractionation
refiners
Pronase
filtrates
enzyme
membrane
filter
Inhibitory Concentration 50

ASJC Scopus subject areas

  • Aquatic Science
  • Biochemistry
  • Molecular Biology
  • Oceanography

Cite this

Fractionation and angiotensin I-converting enzyme (ACE) inhibitory activity of gelatin hydrolysates from by-products of Alaska pollock surimi. / Park, Chan Ho; Kim, Hyung Jun; Kang, Kyung Tae; Park, Jae W.; Kim, Jin Soo.

In: Fisheries and Aquatic Science, Vol. 12, No. 2, 01.06.2009, p. 79-85.

Research output: Contribution to journalArticle

Park, Chan Ho ; Kim, Hyung Jun ; Kang, Kyung Tae ; Park, Jae W. ; Kim, Jin Soo. / Fractionation and angiotensin I-converting enzyme (ACE) inhibitory activity of gelatin hydrolysates from by-products of Alaska pollock surimi. In: Fisheries and Aquatic Science. 2009 ; Vol. 12, No. 2. pp. 79-85.
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