Fruit ripening in sour cherry

Changes in expression of genes encoding expansins and other cell-wall-modifying enzymes

Sang-Dong Yoo, Zhifang Gao, Claudio Cantini, Wayne H. Loescher, Steven Van Nocker

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A preliminary understanding of developmental processes among divergent species is essential to evaluate the applicability of information from model species to plants of agricultural importance. In tomato (Lycopersicon esculentum Mill.), where the molecular biology associated with fruit ripening has been studied most extensively, tissue softening is due at least in part to the activity of proteins called expansins, in concert with enzymatic activities that modify the pectin and xyloglucan components of the cell wall. We evaluated the potential for the concerted action of expansins and other cell wall-modifying enzymes during ripening in a highly divergent fruit species, sour cherry (Prunus cerasus L.). We identified a family of four expansin genes that was strongly upregulated at the advent of ripening. Activation of these genes was accompanied by strong upregulation of gene(s) encoding potential pectin methylesterases, pectate lyase(s), and xyloglucan endotransglycosylase(s). Initiation of ripening and gene induction were also associated with a rapid decrease in cell wall weight. These results suggest that expansin and several other distinct activities could be involved in ripening-associated cell wall modification in cherries.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalJournal of the American Society for Horticultural Science
Volume128
Issue number1
Publication statusPublished - 2003 Jan 1
Externally publishedYes

Fingerprint

expansins
Prunus cerasus
Cell Wall
Fruit
ripening
cell walls
Gene Expression
gene expression
fruits
Enzymes
Lycopersicon esculentum
enzymes
Genes
xyloglucan:xyloglucosyl transferase
pectate lyase
xyloglucans
gene activation
Transcriptional Activation
pectinesterase
Action Potentials

Keywords

  • Expansin
  • Fruit ripening
  • Fruit softening
  • PEL
  • PME
  • Prunus cerasus
  • XET

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Fruit ripening in sour cherry : Changes in expression of genes encoding expansins and other cell-wall-modifying enzymes. / Yoo, Sang-Dong; Gao, Zhifang; Cantini, Claudio; Loescher, Wayne H.; Van Nocker, Steven.

In: Journal of the American Society for Horticultural Science, Vol. 128, No. 1, 01.01.2003, p. 16-22.

Research output: Contribution to journalArticle

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