Abstract
We developed an off-axis quantitative phase microscopy that works for a light source with an extremely short spatial coherence length in order to reduce the diffraction noise and enhance the spatial resolution. A dynamic speckle wave whose coherence length is 440nm was used as an illumination source. To implement an off-axis interferometry for a source of low spatial coherence, a diffraction grating was inserted in the reference beam path. In doing so, an oblique illumination was generated without rotation of the wavefront, which leads to a full-field and single-shot phase recording with improved phase sensitivity of more than a factor of 10 in comparison with coherent illumination. The spatial resolution, both laterally and axially, and the depth selectivity are significantly enhanced due to the wide angular spectrum of the speckle wave. We applied our method to image the dynamics of small intracellular particles in live biological cells. With enhanced phase sensitivity and speed, the proposed method will serve as a useful tool to study the dynamics of biological specimens.
| Original language | English |
|---|---|
| Pages (from-to) | 2465-2467 |
| Number of pages | 3 |
| Journal | Optics Letters |
| Volume | 36 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 2011 Jul 1 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Full-field and single-shot quantitative phase microscopy using dynamic speckle illumination. / Choi, Youngwoon; Yang, Taeseok Daniel; Lee, Kyoung Jin; Choi, Wonshik.
In: Optics Letters, Vol. 36, No. 13, 01.07.2011, p. 2465-2467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Full-field and single-shot quantitative phase microscopy using dynamic speckle illumination
AU - Choi, Youngwoon
AU - Yang, Taeseok Daniel
AU - Lee, Kyoung Jin
AU - Choi, Wonshik
PY - 2011/7/1
Y1 - 2011/7/1
N2 - We developed an off-axis quantitative phase microscopy that works for a light source with an extremely short spatial coherence length in order to reduce the diffraction noise and enhance the spatial resolution. A dynamic speckle wave whose coherence length is 440nm was used as an illumination source. To implement an off-axis interferometry for a source of low spatial coherence, a diffraction grating was inserted in the reference beam path. In doing so, an oblique illumination was generated without rotation of the wavefront, which leads to a full-field and single-shot phase recording with improved phase sensitivity of more than a factor of 10 in comparison with coherent illumination. The spatial resolution, both laterally and axially, and the depth selectivity are significantly enhanced due to the wide angular spectrum of the speckle wave. We applied our method to image the dynamics of small intracellular particles in live biological cells. With enhanced phase sensitivity and speed, the proposed method will serve as a useful tool to study the dynamics of biological specimens.
AB - We developed an off-axis quantitative phase microscopy that works for a light source with an extremely short spatial coherence length in order to reduce the diffraction noise and enhance the spatial resolution. A dynamic speckle wave whose coherence length is 440nm was used as an illumination source. To implement an off-axis interferometry for a source of low spatial coherence, a diffraction grating was inserted in the reference beam path. In doing so, an oblique illumination was generated without rotation of the wavefront, which leads to a full-field and single-shot phase recording with improved phase sensitivity of more than a factor of 10 in comparison with coherent illumination. The spatial resolution, both laterally and axially, and the depth selectivity are significantly enhanced due to the wide angular spectrum of the speckle wave. We applied our method to image the dynamics of small intracellular particles in live biological cells. With enhanced phase sensitivity and speed, the proposed method will serve as a useful tool to study the dynamics of biological specimens.
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UR - http://www.scopus.com/inward/citedby.url?scp=79959977028&partnerID=8YFLogxK
U2 - 10.1364/OL.36.002465
DO - 10.1364/OL.36.002465
M3 - Article
C2 - 21725446
AN - SCOPUS:79959977028
VL - 36
SP - 2465
EP - 2467
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 13
ER -