Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses

Jung Hwa Lee, Seok Won Hyung, Dong Gi Mun, Hee Jung Jung, Hokeun Kim, Hangyeore Lee, Su Jin Kim, Kyong Soo Park, Ronald J. Moore, Richard D. Smith, Sang-Won Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A multifunctional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography or SCX/RPLC) separations and online phosphopeptide enrichment using a single binary nanoflow pump has been developed. With a simple operation of a function selection valve equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses essentially the same solvent flow paths, the same trap column, and the same separation column for reverse-phase separation of 1D, 2D, and online phosphopeptides enrichment experiments, the elution time information obtained from these experiments is in excellent agreement, which facilitates correlating peptide information from different experiments. The final reverse-phase separation of the three experiments is completely decoupled from all of the function selection processes; thereby salts or acids from SCX or TiO2 column do not affect the efficiency of the reverse-phase separation.

Original languageEnglish
Pages (from-to)4373-4381
Number of pages9
JournalJournal of Proteome Research
Volume11
Issue number8
DOIs
Publication statusPublished - 2012 Aug 3

Fingerprint

Phosphopeptides
Liquid chromatography
Proteome
Liquid Chromatography
Pressure
Phase separation
Reverse-Phase Chromatography
Cations
Salts
Experiments
Peptides
Acids
Ion exchange
Pumps
titanium dioxide

Keywords

  • 1DLC
  • 2DLC
  • Bottom-Up Proteomics
  • Multifunctional LC
  • Online Phosphopeptide Enrichment
  • tandem mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses. / Lee, Jung Hwa; Hyung, Seok Won; Mun, Dong Gi; Jung, Hee Jung; Kim, Hokeun; Lee, Hangyeore; Kim, Su Jin; Park, Kyong Soo; Moore, Ronald J.; Smith, Richard D.; Lee, Sang-Won.

In: Journal of Proteome Research, Vol. 11, No. 8, 03.08.2012, p. 4373-4381.

Research output: Contribution to journalArticle

Lee, JH, Hyung, SW, Mun, DG, Jung, HJ, Kim, H, Lee, H, Kim, SJ, Park, KS, Moore, RJ, Smith, RD & Lee, S-W 2012, 'Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses', Journal of Proteome Research, vol. 11, no. 8, pp. 4373-4381. https://doi.org/10.1021/pr3004166
Lee, Jung Hwa ; Hyung, Seok Won ; Mun, Dong Gi ; Jung, Hee Jung ; Kim, Hokeun ; Lee, Hangyeore ; Kim, Su Jin ; Park, Kyong Soo ; Moore, Ronald J. ; Smith, Richard D. ; Lee, Sang-Won. / Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses. In: Journal of Proteome Research. 2012 ; Vol. 11, No. 8. pp. 4373-4381.
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