Fully integrated high-speed intravascular optical coherence tomography/near-infrared fluorescence structural/molecular imaging in vivo using a clinically available near-infrared fluorescence-emitting indocyanine green to detect inflamed lipid-rich atheromata in coronary-sized vessels

Sunki Lee, Min Woo Lee, Han Saem Cho, Joon Woo Song, Hyeong Soo Nam, Dong Joo Oh, Kyeongsoon Park, Wang Yuhl Oh, Hongki Yoo, Jin Won Kim

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background-Lipid-rich inflamed coronary plaques are prone to rupture. The purpose of this study was to assess lipidrich inflamed plaques in vivo using fully integrated high-speed optical coherence tomography (OCT)/near-infrared fluorescence (NIRF) molecular imaging with a Food and Drug Administration-approved indocyanine green (ICG). Methods and Results-An integrated high-speed intravascular OCT/NIRF imaging catheter and a dual-modal OCT/NIRF system were constructed based on a clinical OCT platform. For imaging lipid-rich inflamed plaques, the Food and Drug Administration-approved NIRF-emitting ICG (2.25 mg/kg) or saline was injected intravenously into rabbit models with experimental atheromata induced by balloon injury and 12- to 14-week high-cholesterol diets. Twenty minutes after injection, in vivo OCT/NIRF imaging of the infrarenal aorta and iliac arteries was acquired only under contrast flushing through catheter (pullback speed up to ≤20 mm/s). NIRF signals were strongly detected in the OCT-visualized atheromata of the ICG-injected rabbits. The in vivo NIRF target-to-background ratio was significantly larger in the ICG-injected rabbits than in the saline-injected controls (P<0.01). Ex vivo peak plaque target-to-background ratios were significantly higher in ICG-injected rabbits than in controls (P<0.01) on fluorescence reflectance imaging, which correlated well with the in vivo target-to-background ratios (P<0.01; r=0.85) without significant bias (0.41). Cellular ICG uptake, correlative fluorescence microscopy, and histopathology also corroborated the in vivo imaging findings. Conclusions-Integrated OCT/NIRF structural/molecular imaging with a Food and Drug Administration -approved ICG accurately identified lipid-rich inflamed atheromata in coronary-sized vessels. This highly translatable dual-modal imaging approach could enhance our capabilities to detect high-risk coronary plaques.

Original languageEnglish
Pages (from-to)560-569
Number of pages10
JournalCirculation: Cardiovascular Interventions
Volume7
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

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Molecular Imaging
Indocyanine Green
Optical Coherence Tomography
Atherosclerotic Plaques
Coronary Vessels
Fluorescence
Lipids
Optical Imaging
United States Food and Drug Administration
Rabbits
Catheters
Iliac Artery
Fluorescence Microscopy
Aorta
Rupture
Theoretical Models
Cholesterol
Diet
Injections
Wounds and Injuries

Keywords

  • Atherosclerotic
  • Indocyanine green
  • Molecular imaging
  • Optical coherence tomography
  • Plaque

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Fully integrated high-speed intravascular optical coherence tomography/near-infrared fluorescence structural/molecular imaging in vivo using a clinically available near-infrared fluorescence-emitting indocyanine green to detect inflamed lipid-rich atheromata in coronary-sized vessels. / Lee, Sunki; Lee, Min Woo; Cho, Han Saem; Song, Joon Woo; Nam, Hyeong Soo; Oh, Dong Joo; Park, Kyeongsoon; Oh, Wang Yuhl; Yoo, Hongki; Kim, Jin Won.

In: Circulation: Cardiovascular Interventions, Vol. 7, No. 4, 01.01.2014, p. 560-569.

Research output: Contribution to journalArticle

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abstract = "Background-Lipid-rich inflamed coronary plaques are prone to rupture. The purpose of this study was to assess lipidrich inflamed plaques in vivo using fully integrated high-speed optical coherence tomography (OCT)/near-infrared fluorescence (NIRF) molecular imaging with a Food and Drug Administration-approved indocyanine green (ICG). Methods and Results-An integrated high-speed intravascular OCT/NIRF imaging catheter and a dual-modal OCT/NIRF system were constructed based on a clinical OCT platform. For imaging lipid-rich inflamed plaques, the Food and Drug Administration-approved NIRF-emitting ICG (2.25 mg/kg) or saline was injected intravenously into rabbit models with experimental atheromata induced by balloon injury and 12- to 14-week high-cholesterol diets. Twenty minutes after injection, in vivo OCT/NIRF imaging of the infrarenal aorta and iliac arteries was acquired only under contrast flushing through catheter (pullback speed up to ≤20 mm/s). NIRF signals were strongly detected in the OCT-visualized atheromata of the ICG-injected rabbits. The in vivo NIRF target-to-background ratio was significantly larger in the ICG-injected rabbits than in the saline-injected controls (P<0.01). Ex vivo peak plaque target-to-background ratios were significantly higher in ICG-injected rabbits than in controls (P<0.01) on fluorescence reflectance imaging, which correlated well with the in vivo target-to-background ratios (P<0.01; r=0.85) without significant bias (0.41). Cellular ICG uptake, correlative fluorescence microscopy, and histopathology also corroborated the in vivo imaging findings. Conclusions-Integrated OCT/NIRF structural/molecular imaging with a Food and Drug Administration -approved ICG accurately identified lipid-rich inflamed atheromata in coronary-sized vessels. This highly translatable dual-modal imaging approach could enhance our capabilities to detect high-risk coronary plaques.",
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AU - Lee, Min Woo

AU - Cho, Han Saem

AU - Song, Joon Woo

AU - Nam, Hyeong Soo

AU - Oh, Dong Joo

AU - Park, Kyeongsoon

AU - Oh, Wang Yuhl

AU - Yoo, Hongki

AU - Kim, Jin Won

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