Function of cell-bound and released exopolysaccharides produced by Lactobacillus rhamnosus ATCC 9595

Ji Uk Kim, Younghoon Kim, Kyoung Sik Han, Sejong Oh, Kwang Youn Whang, Jai Neung Kim, Sae Hun Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The physiological characteristics and function of the exopolysalcharide (EPS) produced by Lactobacillus rhamnosus ATCC 9595 were determined. The total quantity of EPS was rapidly increased to 496±20 mg/l during the exponential phase, and then maintained steadily during the stationary phase. During the exponential phase (18 h), the total EPS consisted of 61% cell-bound EPS (cb-EPS) and 39% released EPS (r-EPS), whereas the relative proportion of EPS during the stationary phase (48 h) was converted to 23% cb-EPS and 77% r-EPS. On gel permeation chromatography, cb-EPS was fractionated as a single peak of 8.6 × 106 Da, whereas r-EPS was fractionated as two peaks with average molecular weights of 4.3 × 104 and 8.6 × 106 Da. Interestingly, both EPS species exhibited anticancer properties and cholera toxin-binding activities. Our results suggest that the EPS generated by L. rhamnosus ATCC 9595 might be suitable for use as a functional food or food supplement.

Original languageEnglish
Pages (from-to)939-945
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume16
Issue number6
Publication statusPublished - 2006 Jun 1

Fingerprint

Lactobacillus rhamnosus
Cholera Toxin
Gel permeation chromatography
Molecular weight
Functional Food
Dietary Supplements
Gel Chromatography
Molecular Weight
Functional food

Keywords

  • Anticancer activity
  • Cell-bound EPS
  • Exopolysaccharide
  • Lactobacillus rhamnosus
  • Released EPS
  • Toxin-binding activity

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Function of cell-bound and released exopolysaccharides produced by Lactobacillus rhamnosus ATCC 9595. / Kim, Ji Uk; Kim, Younghoon; Han, Kyoung Sik; Oh, Sejong; Whang, Kwang Youn; Kim, Jai Neung; Kim, Sae Hun.

In: Journal of Microbiology and Biotechnology, Vol. 16, No. 6, 01.06.2006, p. 939-945.

Research output: Contribution to journalArticle

Kim, Ji Uk ; Kim, Younghoon ; Han, Kyoung Sik ; Oh, Sejong ; Whang, Kwang Youn ; Kim, Jai Neung ; Kim, Sae Hun. / Function of cell-bound and released exopolysaccharides produced by Lactobacillus rhamnosus ATCC 9595. In: Journal of Microbiology and Biotechnology. 2006 ; Vol. 16, No. 6. pp. 939-945.
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