Function of cell-bound and released exopolysaccharides produced by Lactobacillus rhamnosus ATCC 9595

Ji Uk Kim, Younghoon Kim, Kyoung Sik Han, Sejong Oh, Kwang Youn Whang, Jai Neung Kim, Sae Hun Kim

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25 Citations (Scopus)

Abstract

The physiological characteristics and function of the exopolysalcharide (EPS) produced by Lactobacillus rhamnosus ATCC 9595 were determined. The total quantity of EPS was rapidly increased to 496±20 mg/l during the exponential phase, and then maintained steadily during the stationary phase. During the exponential phase (18 h), the total EPS consisted of 61% cell-bound EPS (cb-EPS) and 39% released EPS (r-EPS), whereas the relative proportion of EPS during the stationary phase (48 h) was converted to 23% cb-EPS and 77% r-EPS. On gel permeation chromatography, cb-EPS was fractionated as a single peak of 8.6 × 106 Da, whereas r-EPS was fractionated as two peaks with average molecular weights of 4.3 × 104 and 8.6 × 106 Da. Interestingly, both EPS species exhibited anticancer properties and cholera toxin-binding activities. Our results suggest that the EPS generated by L. rhamnosus ATCC 9595 might be suitable for use as a functional food or food supplement.

Original languageEnglish
Pages (from-to)939-945
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume16
Issue number6
Publication statusPublished - 2006 Jun 1

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Keywords

  • Anticancer activity
  • Cell-bound EPS
  • Exopolysaccharide
  • Lactobacillus rhamnosus
  • Released EPS
  • Toxin-binding activity

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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