Function of ezrin-radixin-moesin proteins in migration of subventricular zone-derived neuroblasts following traumatic brain injury

Younghye Moon, Joo Yeon Kim, Woon Ryoung Kim, Hyun Jung Kim, Min Jee Jang, Yoonkey Nam, Kyungjin Kim, Woong Sun

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Throughout life, newly generated neuroblasts from the subventricular zone migrate toward the olfactory bulb through the rostral migratory stream. Upon brain injury, these migrating neuroblasts change their route and begin to migrate toward injured regions, which is one of the regenerative responses after brain damage. This injuryinduced migration is triggered by stromal cell-derived factor 1 (SDF1) released from microglia near the damaged site; however, it is still unclear how these cells transduce SDF1 signals and change their direction. In this study, we found that SDF1 promotes the phosphorylation of ezrinradixin- moesin (ERM) proteins, which are key molecules in organizing cell membrane and linking signals from the extracellular environment to the intracellular actin cytoskeleton. Blockade of ERM activation by overexpressing dominant-negative ERM (DN-ERM) efficiently perturbed the migration of neuroblasts. Considering that DN-ERMexpressing neuroblasts failed to maintain proper migratory cell morphology, it appears that ERM-dependent regulation of cell shape is required for the efficient migration of neuroblasts. These results suggest that ERM activation is an important step in the directional migration of neuroblasts in response to SDF1-CXCR4 signaling following brain injury.

Original languageEnglish
Pages (from-to)1696-1705
Number of pages10
JournalStem Cells
Volume31
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Lateral Ventricles
Chemokine CXCL12
Proteins
Brain Injuries
Cell Shape
Olfactory Bulb
Microglia
Actin Cytoskeleton
Phosphorylation
Cell Membrane
moesin
Traumatic Brain Injury
ezrin
radixin
Brain

Keywords

  • Brain injury
  • ERM proteins
  • Migration
  • Phosphorylation
  • SDF1-CXCR4

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Function of ezrin-radixin-moesin proteins in migration of subventricular zone-derived neuroblasts following traumatic brain injury. / Moon, Younghye; Kim, Joo Yeon; Kim, Woon Ryoung; Kim, Hyun Jung; Jang, Min Jee; Nam, Yoonkey; Kim, Kyungjin; Sun, Woong.

In: Stem Cells, Vol. 31, No. 8, 01.08.2013, p. 1696-1705.

Research output: Contribution to journalArticle

Moon, Younghye ; Kim, Joo Yeon ; Kim, Woon Ryoung ; Kim, Hyun Jung ; Jang, Min Jee ; Nam, Yoonkey ; Kim, Kyungjin ; Sun, Woong. / Function of ezrin-radixin-moesin proteins in migration of subventricular zone-derived neuroblasts following traumatic brain injury. In: Stem Cells. 2013 ; Vol. 31, No. 8. pp. 1696-1705.
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